Descalzo A M, Coto C
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Rev Argent Microbiol. 1989 Jul-Dec;21(3-4):133-40.
A partially purified extract from fresh green leaves of Melia azedarach L prevents the replication of Pseudorabies virus (Suid herpesvirus, (PrV)) in Vero cells. Monolayers of VEro cells were fully protected against PrV CPE when an appropriate concentration of the antiviral compound was added to the culture medium after virus adsorption and remained during 72 h. By contrast, if cells were pretreated with the same amount of antiviral during 18 h before infection, CPE produced by PrV was only slightly reduced. The antiviral activity of the compound specifically affect PrV multiplication since it was found that no toxic effects on cells were observed at the concentrations assayed which inhibited virus yield by 99%. The effect or the antiviral on adsorption or penetration steps was investigated. Neither the number or viral particles that penetrate nor the amount of unadsorbed virus were the same in treated and non-treated cultures. In order to determine whether an early of late step of intracellular virus replication was inhibited, the extract was added at different times post-infection and the virus yield was determined. The production of infectious virus decreased almost 3 log when the compound was added up to 4 h. p.i. According to these results we postulate that an early stage of virus replication, probably beta-messenger expression is inhibited.
从苦楝新鲜绿叶中提取的部分纯化提取物可抑制伪狂犬病病毒（猪疱疹病毒，PrV）在Vero细胞中的复制。当在病毒吸附后向培养基中加入适当浓度的抗病毒化合物并维持72小时时，Vero细胞单层可完全免受PrV细胞病变效应（CPE）的影响。相比之下，如果在感染前18小时用相同量的抗病毒药物预处理细胞，PrV产生的CPE仅略有降低。该化合物的抗病毒活性特异性地影响PrV的增殖，因为发现在测定的浓度下对细胞没有毒性作用，而这些浓度可使病毒产量降低99%。研究了该抗病毒药物对吸附或穿透步骤的影响。在处理过的和未处理的培养物中，穿透的病毒颗粒数量和未吸附的病毒量均不相同。为了确定细胞内病毒复制的早期或晚期步骤是否受到抑制，在感染后不同时间加入提取物并测定病毒产量。当在感染后4小时内加入该化合物时，感染性病毒的产生减少了近3个对数。根据这些结果，我们推测病毒复制的早期阶段，可能是β信使表达受到了抑制。
