Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico, Químicas y Naturales de la Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Argentina.
Nat Prod Res. 2012;26(17):1621-5. doi: 10.1080/14786419.2011.576394. Epub 2011 Oct 17.
The methanolic extract of Verbascum thapsus was evaluated for its antiviral activity against the pseudorabies virus strain RC/79 (PrV), and also for its cytotoxic activity on Vero cells. The extract showed CC₅₀ values of 1100 µg mL⁻¹ and 1426 µg mL⁻¹ by NRU and MTT assays, respectively. The 50% inhibitory concentration of the extract for PrV plaque formation was determined at 35 µg mL⁻¹, and selectivity indices were 31.4 (NRU) and 40.7 (MTT). When cells were pre-treated with the extract prior to virus infection, the inhibition in plaque formation was 70%. PrV was highly inhibited when it was incubated with plant extract or when the extract was added during the adsorption phase (99%). However, no inhibitory effect was observed when the extract was added to the cells after the adsorption period. Thus, these results suggest that the methanolic extract of Verbascum thapsus may contain bioactive compound(s) that affect PrV mostly in the adsorption phase.
龙葵的甲醇提取物被评估了其抗伪狂犬病毒株 RC/79（PrV）的抗病毒活性，也评估了其对 Vero 细胞的细胞毒性活性。提取物通过 NRU 和 MTT 试验分别显示出 CC₅₀ 值为 1100µg/mL 和 1426µg/mL。提取物对 PrV 蚀斑形成的 50%抑制浓度为 35µg/mL，选择性指数为 31.4（NRU）和 40.7（MTT）。当细胞在用病毒感染之前用提取物预处理时，对蚀斑形成的抑制率为 70%。当 PrV 与植物提取物孵育或在吸附阶段加入提取物时，PrV 受到高度抑制（99%）。然而，当吸附期后将提取物加入细胞时，没有观察到抑制作用。因此，这些结果表明，龙葵的甲醇提取物可能含有生物活性化合物，这些化合物主要在吸附阶段影响 PrV。
