荧光大环-染料复合物的缔合化学传感——超越指示剂置换的通用酶分析平台。
Associative chemosensing by fluorescent macrocycle-dye complexes – a versatile enzyme assay platform beyond indicator displacement.
作者信息
Biedermann Frank, Hathazi Denisa, Nau Werner M
机构信息
Department of Life Sciences and Chemistry, Jacobs University Bremen, Campus Ring 1, 28759 Bremen, Germany.
出版信息
Chem Commun (Camb). 2015 Mar 25;51(24):4977-80. doi: 10.1039/c4cc10227d.
PMID:25622263
Abstract
A label-free in situ method to monitor reactions in real time by using fluorescent supramolecular chemosensors based on cucurbit[8]uril is presented. It allows sensing of enzymatic activity, inhibitor and activator screening, and analyte detection with unprecedented versatility and high sensitivity.
摘要
本文提出了一种基于葫芦[8]脲的荧光超分子化学传感器实时监测反应的无标记原位方法。它能够以前所未有的多功能性和高灵敏度实现酶活性检测、抑制剂和激活剂筛选以及分析物检测。
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