Department of Life Sciences and Chemistry, Jacobs University Bremen, Campus Ring 1, 28759, Bremen, Germany.
Chem Commun (Camb). 2019 Jan 10;55(5):671-674. doi: 10.1039/c8cc09126a.
A supramolecular host-guest FRET pair based on a carboxyfluorescein-labelled cucurbit[7]uril (CB7-CF, as acceptor) and the fluorescent dye 4',6-diamidino-2-phenylindole (DAPI, as donor) is developed for sensing of DNA. In comparison to the commercial DNA staining dye SYBR Green I, the new chemosensing ensemble offers dual-emission signals, which allows a linear ratiometric response over a wide concentration range.
开发了一种基于羧基荧光素标记的葫芦[7]脲（CB7-CF，作为受体）和荧光染料 4'，6-二脒基-2-苯基吲哚（DAPI，作为供体）的超分子主客体荧光能量转移对，用于 DNA 传感。与商业 DNA 染色染料 SYBR Green I 相比，新的化学传感组合提供了双发射信号，这使得在很宽的浓度范围内可以进行线性比率响应。
