食管鳞状细胞癌中P27、脆性组氨酸三联体（FHT）、第10号染色体缺失的磷酸酶和张力蛋白同源物（PTEN）、P73的表达与预后的关系

Relationship between expression of P27, Fragile Histidine Triad (FHT), phosphatase and tensin homolog deleted on chromosome ten (PTEN), P73, and prognosis in esophageal squamous cell carcinoma.

作者信息

Chen Yanning, Wang Xiaoling, Li Fang, Zhang Lingling, Ma Li, Liu Yueping

机构信息

Department of Pathology, Institute of the Fourth Hospital of Hebei Medical University, No. 12, Jiankangt Road, Shijiazhuang, 050011, PR China.

Department of Surgery, Institute of the Fourth Hospital of Hebei Medical University, No. 12, Jiankangt Road, Shijiazhuang, 050011, PR China.

出版信息

Ann Diagn Pathol. 2015 Feb;19(1):33-6. doi: 10.1016/j.anndiagpath.2014.12.004. Epub 2014 Dec 29.

DOI:10.1016/j.anndiagpath.2014.12.004

PMID:25622532

Abstract

To investigate the significance between the expression of P27, Fragile Histidine Triad (FHIT), phosphatase and tensin homolog deleted on chromosome ten (PTEN), and P73 in esophageal squamous cell carcinoma (ESCC), paraffin-embedded tissue blocks of 200 cases were obtained from the Fourth Hospital of Hebei Medical University. The sections were used for (HE) and immunohistochemical staining streptavidin-perosidase (SP). The immunologic reagents used included antibodies against P27, FHIT, PTEN, and P73. From I- to II- and III-graded ESCC, the positive expression of P27 was decreased, but the P73 was increased, showing a ladded change (P < .05). The oncogene and tumor suppressor gene protein expression were related to the differentiation and can be one of the factors of influencing prognosis. The oncogene and tumor suppressor gene protein expression was related to the prognostic factor, and thus, it is valuable for clinical treatment and judging prognosis to detect the expression of P27, FHIT, PTEN, and P73 in ESCC.

摘要

为研究P27、脆性组氨酸三联体（FHIT）、第10号染色体缺失的磷酸酶和张力蛋白同源物（PTEN）以及P73在食管鳞状细胞癌（ESCC）中的表达意义，从河北医科大学第四医院获取了200例石蜡包埋组织块。切片用于苏木精-伊红（HE）染色和免疫组织化学链霉亲和素-过氧化物酶（SP）染色。所用免疫试剂包括抗P27、FHIT、PTEN和P73抗体。从I级到II级和III级ESCC，P27的阳性表达降低，而P73增加，呈阶梯状变化（P <.05）。癌基因和抑癌基因蛋白表达与分化相关，可能是影响预后的因素之一。癌基因和抑癌基因蛋白表达与预后因素相关，因此，检测ESCC中P27、FHIT、PTEN和P73的表达对临床治疗和判断预后具有重要价值。