Ye Hui, Guo Xia
Department of Obstetrics, Baoji Hospital of Traditional Chinese Medicine, Baoji City, Shaanxi Province 721001, China.
Department of Obstetrics and Gynezcology, Hanjiang Hospital Affiliated to Xi'an Medical College, Hanzhong City, Shaanxi Province 723003, China
Biosci Rep. 2019 Aug 5;39(8). doi: 10.1042/BSR20190095. Print 2019 Aug 30.
Tumor protein p73 (TP73) has been reported to be dysregulated in various types of human cancer and associated with clinical progression and outcome. Owing to the lack of reports on the correlation between TP73 protein expression and clinicopathologic features of cervical cancer, the aim of our research was to explore the clinical and prognostic significance of TP73 protein expression in cervical cancer patients. In our study, TP73 protein expression was detected by immunochemistry in 118 paraffin-embedded cervical cancer tissue specimens and 40 paraffin-embedded normal cervical epithelium tissue specimens. In the results, we found cervical cancer tissues exhibited high TP73 expression in comparison with normal cervical epithelium tissues, which was consistent with the expression status of TP73 in The Cancer Genome Atlas (TCGA) database. Furthermore, we analyzed the relationships between TP73 expression and clinicopathologic features through using the chi-square test or Fisher's exact test, and found high expression of TP73 was markedly associated with early clinical stage, less lymph node metastasis, absent distant metastasis, squamous cell carcinoma and favorable histological grade. The Kaplan-Meier method and log-rank test were performed based on the expression level of TP73 in a cervical cancer cohort from the TCGA database, and showed that TP73 expression was positively correlated with overall survival time in cervical cancer patients. Moreover, univariate and multivariate Cox proportional hazards regression model indicated that high TP73 expression was identified as an independent factor for predicting favorable overall survival in cervical cancer patients. In conclusion, TP73 expression is increased in cervical cancer tissues and cells, and acts as a credible biomarker for predicting favorable overall survival in cervical cancer patients.
据报道，肿瘤蛋白p73（TP73）在多种人类癌症中表达失调，并与临床进展和预后相关。由于缺乏关于TP73蛋白表达与宫颈癌临床病理特征之间相关性的报道，我们研究的目的是探讨TP73蛋白表达在宫颈癌患者中的临床和预后意义。在我们的研究中，采用免疫化学方法检测了118例石蜡包埋的宫颈癌组织标本和40例石蜡包埋的正常宫颈上皮组织标本中的TP73蛋白表达。结果发现，与正常宫颈上皮组织相比，宫颈癌组织中TP73表达较高，这与癌症基因组图谱（TCGA）数据库中TP73的表达状态一致。此外，我们通过卡方检验或Fisher精确检验分析了TP73表达与临床病理特征之间的关系，发现TP73高表达与临床早期、较少的淋巴结转移、无远处转移、鳞状细胞癌和良好的组织学分级显著相关。基于TCGA数据库中一组宫颈癌患者的TP73表达水平，采用Kaplan-Meier法和对数秩检验，结果显示TP73表达与宫颈癌患者的总生存时间呈正相关。此外，单因素和多因素Cox比例风险回归模型表明，TP73高表达被确定为预测宫颈癌患者良好总生存的独立因素。总之，宫颈癌组织和细胞中TP73表达增加，并且是预测宫颈癌患者良好总生存的可靠生物标志物。
