[血浆循环DNA中Septin9基因甲基化检测在结直肠癌筛查中的意义]

[Significance of Septin9 gene methylation detection of plasma circulation DNA in colorectal cancer screening].

作者信息

Kang Qian, Jin Peng, Yang Lang, Wang Xin, An Hejuan, Liu Lili, Li Na, Sheng Jianqiu

机构信息

Department of Gastroenterology, the Military General Hospital of Beijing, Beijing 100700, China.

Department of Gastroenterology, the Military General Hospital of Beijing, Beijing 100700, China. Email:

出版信息

Zhonghua Yi Xue Za Zhi. 2014 Dec 30;94(48):3839-41.

PMID:25623318

Abstract

OBJECTIVE

To explore the role of detecting the methylation status of gene Septin9 (SEPT9) in plasma for colorectal cancer screening in Chinese population.

METHODS

Patients were collected from Beijing Military General Hospital since September 2013 to February 2014. The performance of SEPT9 assay was validated in a single-blind study of 80 cases with colonoscopy and pathologically verified colorectal cancer and 52 normal controls. The detection of Septin9 gene methylation in peripheral blood was performed by fluorescence quantitative polymerase chain reaction (PCR). And immunoassay fecal occult blood test was conducted to compare the superiority of methylated Septin9 for screening colorectal cancer.

RESULTS

The Septin9 assay successfully identified 75.0% (95%CI:64.7%-83.6%) of cancers at a specificity of 98.1% (95%CI:90.9%-99.9%). And it was superior to fecal occult blood screening for colorectal cancer (sensitivity 79.5% vs 53.8%, P < 0.05).

CONCLUSION

Determination of SEPT9 methylation status is an innovative non-invasive plasma screening test for colorectal cancer.

摘要

目的

探讨检测血浆中Septin9（SEPT9）基因甲基化状态在中国人群结直肠癌筛查中的作用。

方法

收集2013年9月至2014年2月在北京军区总医院就诊的患者。在一项单盲研究中，对80例经结肠镜检查及病理证实的结直肠癌患者和52例正常对照进行SEPT9检测性能验证。采用荧光定量聚合酶链反应（PCR）检测外周血中Septin9基因甲基化。并进行免疫法粪便潜血试验，以比较甲基化Septin9在结直肠癌筛查中的优势。