[倍他司汀对豚鼠单侧迷路切除术后前庭内侧核组胺H3受体表达的影响]

[Influence of betahistine on the expression of histamine H3 receptor in the medial vestibular nucleus following unilateral labyrinthectomy in guinea pigs].

作者信息

Shu Jingcheng, Yin Shihua, Liu Yuan, Rao Xiuli, Wei Shunlian

机构信息

Deparrment of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China.

Deparrment of Otorhinolaryngology Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China. Email:

出版信息

Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2014 Dec;49(12):1027-30.

PMID:25623873

Abstract

OBJECTIVE

To observe the influence of betahistine on the expression of histamine H3 receptor in the medial vestibular nucleus (MVN) following unilateral labyrinthectomy (UL).

METHODS

Fifty-six healthy guinea pigs were randomly divided into three groups:the sham-operated group (group I), the UL group[group II, and UL+betahistine (BET) group (group III)], BET was intraperitoneally injection at 2.17 mg×kg(-1)×d(-1) for 7 days. The expression of histamine H3 receptor was analyzed by immunohistochemistry at 1 day, 3 days and 7 days after UL.

RESULTS

H3 receptors were presented in the MVN and the expression of histamine H3 receptor were increased significantly in the ipsilateral MVN at 1 and 3 days after UL(P < 0.05), the change turned into the normal value at 7 days(P > 0.05). In the UL+BET group, the intensity of histamine H3 receptor was lower than that in the UL at 1 day and 3 days(4.25 ± 0.71, 3.50 ± 0.92 vs 5.75 ± 0.71, 5.50 ± 0.93, P < 0.05). However, the changes turned into the normal values at 3 and 7 days (P > 0.05).

CONCLUSIONS

The early stage of the vestibular compensation process may be associated with the change of H3 receptor expression in MVN. In the UL+BET group the histamine H3 receptor recovered quickly.

摘要

目的

观察倍他司汀对单侧迷路切除（UL）术后前庭内侧核（MVN）中组胺H3受体表达的影响。

方法

56只健康豚鼠随机分为三组：假手术组（I组）、UL组（II组）和UL+倍他司汀（BET）组（III组），BET按2.17 mg×kg⁻¹×d⁻¹腹腔注射，共7天。在UL术后1天、3天和7天，采用免疫组化法分析组胺H3受体的表达。

结果

H3受体在前庭内侧核表达，UL术后1天和3天，同侧前庭内侧核组胺H3受体表达显著增加（P<0.05），7天时恢复至正常水平（P>0.05）。在UL+BET组，1天和3天时组胺H3受体表达强度低于UL组（4.25±0.71，3.50±0.92比5.75±0.71，5.50±0.93，P<0.05）。然而，3天和7天时变化恢复至正常水平（P>0.05）。