单侧迷路破坏后大鼠前庭内侧核和绒球小结叶中 mGluR2 和 mGluR7 表达的变化。
The changes in mGluR2 and mGluR7 expression in rat medial vestibular nucleus and flocculus following unilateral labyrinthectomy.
机构信息
Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Avenue, Wuhan 430022, China.
出版信息
Int J Mol Sci. 2013 Nov 20;14(11):22857-75. doi: 10.3390/ijms141122857.
It is known that the medial vestibular nucleus (MVN) and the cerebellar flocculus are the key areas, which contribute to the behavioral recovery ("vestibular compensation") after unilateral labyrinthectomy (UL). In these areas, how the genetic activities of the metabotropic glutamate receptors mGluR2 and mGluR7 performance after UL is unknown. With the means of quantitative real-time PCR, Western blotting, and immunohistochemistry, we analyzed the expression of mGluR2 and mGluR7 in the bilateral MVN and the flocculus of rats in different stages after UL (the 1st, 3rd, and 7th day). Our results show that in the MVN, the mRNA, and protein expressions of mGluR7 were ipsilaterally decreased at the 1st day following UL. However, in the MVN, no change was observed in the mRNA and protein expressions of mGluR2. On the other hand, the mRNA and protein expression of mGluR2 were enhanced in the ipsilateral flocculus at the 1st day following UL, while in the flocculus no change was shown in mGluR7 mRNA and protein expressions. Our results suggest that mGluR2 and mGluR7 may contribute to the early rebalancing of spontaneous resting activity in the MVN.
已知内侧前庭核（MVN）和小脑绒球是促进单侧迷路切除（UL）后行为恢复（“前庭代偿”）的关键区域。在这些区域，代谢型谷氨酸受体 mGluR2 和 mGluR7 的遗传活性在 UL 后如何变化尚不清楚。我们通过定量实时 PCR、Western blot 和免疫组织化学分析了 UL 后不同时间点（第 1、3 和 7 天）大鼠双侧 MVN 和绒球中 mGluR2 和 mGluR7 的表达。结果显示，在 MVN 中，UL 后第 1 天 mGluR7 的 mRNA 和蛋白表达均出现同侧减少。然而，在 MVN 中，mGluR2 的 mRNA 和蛋白表达没有变化。另一方面，UL 后第 1 天，同侧绒球中 mGluR2 的 mRNA 和蛋白表达增强，而绒球中 mGluR7 的 mRNA 和蛋白表达没有变化。我们的结果表明，mGluR2 和 mGluR7 可能有助于早期重新平衡 MVN 中的自发性静息活动。
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