Zhang Li, Wang Chaozhan, Wei Yinmao
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an, 710069, People's Republic of China.
Biomed Chromatogr. 2015 Aug;29(8):1133-6. doi: 10.1002/bmc.3424. Epub 2015 Jan 26.
Boronate affinity has attracted much attention in recent years. It has been broadly used for selective isolation and enrichment of cis-diol-containing molecules. Conventionally, the cis-diols are adsorbed in mild alkaline aqueous solutions. In this work, for the first time, we found that boronate affinity adsorption could also be performed in nonaqueous solvent at nonbasic pH. Cis-diol-containing compounds present in herbal medicines were used for the adsorption test. The results indicated that all compounds obtained higher recoveries in the organic solvents (methanol, acetonitrile, ethyl acetate) compared with alkaline buffer. The adsorption of vicinal cis-diol-containing molecules in organic solvents could be accomplished rapidly, with high selectivity and high recoveries (>80%). These results shed light on the possibility of boronate affinity adsorption in nonaqueous solvents. The results are very important for the isolation and enrichment of cis-diols, which have poor solubility in water, especially for those in herbal medicines.
近年来，硼酸酯亲和作用备受关注。它已被广泛用于含顺式二醇分子的选择性分离和富集。传统上，顺式二醇在弱碱性水溶液中被吸附。在这项工作中，我们首次发现硼酸酯亲和吸附也可以在非水溶剂中、非碱性pH条件下进行。用于吸附测试的是草药中存在的含顺式二醇的化合物。结果表明，与碱性缓冲液相比，所有化合物在有机溶剂（甲醇、乙腈、乙酸乙酯）中的回收率更高。含邻位顺式二醇分子在有机溶剂中的吸附可以快速完成，具有高选择性和高回收率（>80%）。这些结果揭示了在非水溶剂中进行硼酸酯亲和吸附的可能性。这些结果对于分离和富集在水中溶解度较差的顺式二醇非常重要，尤其是对于草药中的顺式二醇。
