Li Qianjin, Liu Zhen
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Hankou Road 22, Nanjing, 210093, China.
Methods Mol Biol. 2015;1286:159-69. doi: 10.1007/978-1-4939-2447-9_13.
Boronate affinity monolithic columns have been developed into an important means for the selective recognition and capture of cis-diol-containing biomolecules, such as glycoproteins, nucleosides and saccharides. The ligands of boronic acids are playing an important role in boronate affinity monolithic columns. Although several boronate affinity monoliths with high affinity toward cis-diol-containing biomolecules have been reported, only few publications are focused on their detailed procedures for preparation and characterization. This chapter describes in detail the preparation and characterization of a boronate affinity monolithic column applying 2,4-difluoro-3-formyl-phenylboronic acid (DFFPBA) as a ligand. The DFFPBA-functionalized monolithic column not only exhibited an ultrahigh boronate affinity toward cis-diol-containing biomolecules, but also showed great potential for the selective enrichment of cis-diol-containing biomolecules in real samples.
硼酸酯亲和整体柱已发展成为选择性识别和捕获含顺式二醇生物分子（如糖蛋白、核苷和糖类）的重要手段。硼酸配体在硼酸酯亲和整体柱中起着重要作用。尽管已经报道了几种对含顺式二醇生物分子具有高亲和力的硼酸酯亲和整体柱，但只有少数出版物关注其详细的制备和表征方法。本章详细描述了以2,4-二氟-3-甲酰基苯硼酸（DFFPBA）为配体制备硼酸酯亲和整体柱及其表征。DFFPBA功能化整体柱不仅对含顺式二醇生物分子表现出超高的硼酸酯亲和力，而且在实际样品中选择性富集含顺式二醇生物分子方面也显示出巨大潜力。
