用于橄榄油中 AFB1 检测的高灵敏电化学阻抗光谱免疫传感器。
Highly sensitive electrochemical impedance spectroscopy immunosensor for the detection of AFB1 in olive oil.
机构信息
The Education Ministry Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Department of Chemistry, Shanghai Normal University, Shanghai 200234, China.
The Education Ministry Key Laboratory of Resource Chemistry and Shanghai Key Laboratory of Rare Earth Functional Materials, Department of Chemistry, Shanghai Normal University, Shanghai 200234, China.
出版信息
Food Chem. 2015 Jun 1;176:22-6. doi: 10.1016/j.foodchem.2014.12.030. Epub 2014 Dec 18.
Aflatoxin produced by Aspergillus flavus and Aspergillus parasiticus are commonly found in olive and its derivatives. Aflatoxin B1 (AFB1) is a predominant toxin detected abundantly and has been implicated in the etiology of human hepatocellular carcinoma. This study proposes a sensitive and convenient electrochemical impedance spectroscopy (EIS) method for determining AFB1 by MWCNTs/RTIL composite films-based immunosensor. The calibration curve for AFB1 was linear in the range of 0.1-10ngmL(-1) with the limit of detection (LOD) 0.03ngmL(-1). The presence of MWCNTs warrant fast electron transfer, and the ionic liquid provides a benign microenvironment for antibody. The experimental parameters, such as pH and incubating time, have been investigated and optimized. Furthermore, the detection of AFB1 is presented to test this method after extracted from olive oils. It can be anticipated that this method would be used for the detection of AFB1 in various agriculture products and vegetable oils.
黄曲霉和寄生曲霉产生的黄曲霉毒素通常存在于橄榄及其衍生物中。黄曲霉毒素 B1（AFB1）是一种主要的毒素，大量存在，并与人类肝细胞癌的病因有关。本研究提出了一种基于 MWCNTs/RTIL 复合膜的免疫传感器的灵敏、便捷的电化学阻抗谱（EIS）方法来测定 AFB1。AFB1 的校准曲线在 0.1-10ngmL(-1) 范围内呈线性，检测限（LOD）为 0.03ngmL(-1)。MWCNTs 保证了快速的电子转移，而离子液体则为抗体提供了一个良性的微环境。实验参数，如 pH 值和孵育时间，已经被研究和优化。此外，从橄榄油中提取 AFB1 后，对其进行了检测，以验证该方法的可行性。可以预期，该方法将用于检测各种农产品和植物油中的 AFB1。
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