基于 AuNPs/Zn/Ni-ZIF-8-800@graphene 的电化学免疫传感器用于快速检测花生油中的黄曲霉毒素 B1。

Electrochemical immunosensor based on AuNPs/Zn/Ni-ZIF-8-800@graphene for rapid detection of aflatoxin B1 in peanut oil.

机构信息

College of Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, Henan, China; International Joint Research Center for Animal Immunology, Zhengzhou, 450002, Henan, China; Key Laboratory of Nutrition and Healthy Food of Zhengzhou, Zhengzhou, 450002, China.

College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, Henan, China; International Joint Research Center for Animal Immunology, Zhengzhou, 450002, Henan, China; Key Laboratory of Nutrition and Healthy Food of Zhengzhou, Zhengzhou, 450002, China.

出版信息

Anal Biochem. 2022 Aug 1;650:114710. doi: 10.1016/j.ab.2022.114710. Epub 2022 May 11.

DOI:10.1016/j.ab.2022.114710

PMID:35568158

Abstract

Peanut oil is a basic food raw material in life. However, aflatoxin contamination in peanut oil is considered to be one of the most serious food safety problems in the world. Based on AuNPs/Zn/Ni-ZIF-8-800@graphene composite, a simple, efficient and sensitive electrochemical immunosensor was developed to detect aflatoxin B1 (AFB1) in peanut oil. The bare glassy carbon electrode was modified by graphene, bimetallic organic framework material (Zn/Ni-ZIF-8-800), chitosan and gold nanoparticles. The electrochemical immunosensor was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), Fourier transform infrared (FT-IR) and cyclic voltammetry (CV), and the electrochemical signal changes after antibody and AFB1 binding were investigated in detail. Under the optimal conditions, the linear range of the electrochemical immunosensor was 0.18-100 ng/mL, and the detection limit was 0.18 ng/mL. In addition, the prepared sensor has high selectivity and long-term stability, which lays a foundation for the simple, rapid and sensitive detection of AFB1 in peanut oil.

摘要

花生油是生活中的基本食品原料。然而，花生油中的黄曲霉毒素污染被认为是世界上最严重的食品安全问题之一。基于 AuNPs/Zn/Ni-ZIF-8-800@graphene 复合材料，开发了一种简单、高效、灵敏的电化学免疫传感器来检测花生油中的黄曲霉毒素 B1（AFB1）。裸玻碳电极通过石墨烯、双金属有机骨架材料（Zn/Ni-ZIF-8-800）、壳聚糖和金纳米粒子进行修饰。通过扫描电子显微镜（SEM）、X 射线衍射（XRD）、X 射线光电子能谱（XPS）、傅里叶变换红外（FT-IR）和循环伏安法（CV）对电化学免疫传感器进行了表征，并详细研究了抗体和 AFB1 结合后的电化学信号变化。在最佳条件下，电化学免疫传感器的线性范围为 0.18-100ng/mL，检测限为 0.18ng/mL。此外，所制备的传感器具有高选择性和长期稳定性，为简单、快速、灵敏地检测花生油中的 AFB1 奠定了基础。