School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China; School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA.
School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, PR China.
Biosens Bioelectron. 2018 Jun 15;108:69-75. doi: 10.1016/j.bios.2018.02.043. Epub 2018 Mar 21.
Aflatoxin B1 (AFB1), one of the most common mycotoxins in food matrixes, has been identified as the most toxic contaminant with mutagenic, teratogenic, immunosuppressive, and carcinogenic effects. In this work, a magnetically assembled aptasensing device has been designed for label-free determination of AFB1 by employing a disposable screen-printed carbon electrode (SPCE) covered with a designed polydimethylsiloxane (PDMS) film as the micro electrolytic cell. The magnetically controlled bio-probes were firstly prepared by immobilization of the thiolated aptamers on the FeO@Au magnetic beads, which was rapidly assembled on the working electrode of SPCE within 10 s, by using a magnet placed at the opposite side. The PDMS film with a centered hole was covered on the SPCE surface to achieve a more practicable and flexible electrochemical measurement. In this effort, a label-free aptasensor for the sensitive and selective determination of AFB1 has been developed using electrochemical impedance spectroscopy upon the biorecognition between aptamers and the targets. The developed method had a wide linear range of 20 pg mL-50 ng mL with a detection limit of 15 pg mL (S/N = 3) and succeeded in spiked samples of peanuts. The developed aptasensing device shows fantastic application prospect with simple design, easy operation, low cost, and high sensitivity and selectivity characteristics. This sensing strategy represents a promising path toward routine quality control of food safety and creates the opportunity to develop facile aptasensing device for other targets.
黄曲霉毒素 B1（AFB1）是食品基质中最常见的霉菌毒素之一，被认为是最具毒性的污染物，具有致突变、致畸、免疫抑制和致癌作用。在这项工作中，设计了一种基于磁组装的适体传感装置，通过使用覆盖有设计的聚二甲基硅氧烷（PDMS）膜的一次性丝网印刷碳电极（SPCE）作为微电解池，实现了对 AFB1 的无标记测定。磁控生物探针首先通过将巯基化适体固定在 FeO@Au 磁性珠上进行制备，然后通过将磁铁放置在相对侧，将其在 10 s 内快速组装到 SPCE 的工作电极上。在 SPCE 表面覆盖带有中心孔的 PDMS 膜，以实现更实用和灵活的电化学测量。在这项工作中，使用电化学阻抗光谱法，通过适体与目标物之间的生物识别，开发了一种用于灵敏和选择性测定 AFB1 的无标记适体传感器。所开发的方法具有 20 pg mL-50 ng mL 的宽线性范围，检测限为 15 pg mL（S/N = 3），并成功应用于花生的加标样品。所开发的适体传感装置具有设计简单、操作方便、成本低、灵敏度和选择性高的特点，具有广阔的应用前景。这种传感策略代表了食品安全常规质量控制的一种有前途的途径，并为开发其他目标物的简便适体传感装置创造了机会。
