Wang Xianying, Wu Honghai, Xue Gai, Hou Yanning
Hebei Medical University, Shijiazhuang 050017, Hebei Province, China ; The Third Hospital of Hebei Medical University, Shijiazhuang 050051, Hebei Province, China.
Bethune International Peace Hospital of Chinese PLA, Shijiazhuang 050082, Hebei Province, China.
Neural Regen Res. 2012 Sep 5;7(25):1925-30. doi: 10.3969/j.issn.1673-5374.2012.25.001.
In this study, human umbilical cord mesenchymal stem cells from full-term neonates born by vaginal delivery were cultured in medium containing 150 mg/mL of brain tissue extracts from Sprague-Dawley rats (to mimic the brain microenvironment). Immunocytochemical analysis demonstrated that the cells differentiated into neuron-like cells. To evaluate the effects of progesterone as a neurosteroid on the neuronal differentiation of human umbilical cord mesenchymal stem cells, we cultured the cells in medium containing progesterone (0.1, 1, 10 μM) in addition to brain tissue extracts. Reverse transcription-PCR and flow cytometric analysis of neuron specific enolase-positive cells revealed that the percentages of these cells increased significantly following progesterone treatment, with the optimal progesterone concentration for neuron-like differentiation being 1 μM. These results suggest that progesterone can enhance the neuronal differentiation of human umbilical cord mesenchymal stem cells in culture medium containing brain tissue extracts to mimic the brain microenvironment.
在本研究中，将经阴道分娩的足月新生儿的人脐带间充质干细胞培养于含有150 mg/mL来自Sprague-Dawley大鼠的脑组织提取物的培养基中（以模拟脑微环境）。免疫细胞化学分析表明这些细胞分化为神经元样细胞。为了评估作为神经甾体的孕酮对人脐带间充质干细胞神经元分化的影响，我们除了在含有脑组织提取物的培养基中培养细胞外，还在含有孕酮（0.1、1、10 μM）的培养基中培养细胞。对神经元特异性烯醇化酶阳性细胞进行逆转录聚合酶链反应和流式细胞术分析发现，孕酮处理后这些细胞的百分比显著增加，神经元样分化的最佳孕酮浓度为1 μM。这些结果表明，在含有脑组织提取物以模拟脑微环境的培养基中，孕酮可增强人脐带间充质干细胞的神经元分化。
