Zhang Sai, Liu Xiao-zhi, Liu Zhen-lin, Wang Yan-min, Hu Qun-liang, Ma Tie-zhu, Sun Shi-zhong
Department of Neurosurgery, Affiliated Hospital of Medical College of Chinese People's Armed Police Force, Tianjin, China.
Chin J Traumatol. 2009 Aug;12(4):195-9.
To promote stem cells differentiation into neurons and enhance neuromotor function after brain injury through brain-derived neurotrophic factor (BDNF) induction.
Recombinant adenovirus vector was applied to the transfection of BDNF into human-derived umbilical cord mesenchymal stem cells (UCMSCs). Enzyme linked immunosorbent assay (ELISA) was used to determine the secretion phase of BDNF. The brain injury model of athymic mice induced by hydraulic pressure percussion was established for transplantation of stem cells into the edge of injury site. Nerve function scores were obtained, and the expression level of transfected and non-transfected BDNF, proportion of neuron specific enolase (NSE) and glial fibrillary acidic protein (GFAP), and the number of apoptosis cells were compared respectively.
The BDNF expression achieved its stabilization at a high level 72 hours after gene transfection. The mouse obtained a better score of nerve function, and the proportion of the NSE-positive cells increased significantly (P<0.05), but GFAP-positive cells decreased in BDNF-UCMSCs group compared with the other two groups (P<0.05). At the site of high expression of BDNF, the number of apoptosis cells decreased markedly.
BDNF gene can promote the differentiation of the stem cells into neurons rather than glial cells, and enhance neuromotor function after brain injury.
通过脑源性神经营养因子（BDNF）诱导，促进脑损伤后干细胞向神经元分化并增强神经运动功能。
应用重组腺病毒载体将BDNF转染至人脐带间充质干细胞（UCMSCs）。采用酶联免疫吸附测定（ELISA）法测定BDNF的分泌阶段。建立液压冲击诱导的无胸腺小鼠脑损伤模型，将干细胞移植到损伤部位边缘。获得神经功能评分，分别比较转染和未转染BDNF的表达水平、神经元特异性烯醇化酶（NSE）和胶质纤维酸性蛋白（GFAP）的比例以及凋亡细胞数量。
基因转染后72小时，BDNF表达达到高水平并稳定。与其他两组相比，BDNF-UCMSCs组小鼠神经功能评分更好，NSE阳性细胞比例显著增加（P<0.05），但GFAP阳性细胞减少（P<0.05）。在BDNF高表达部位，凋亡细胞数量明显减少。
BDNF基因可促进干细胞向神经元而非胶质细胞分化，并增强脑损伤后的神经运动功能。
