Yu Wei, Wang Jian, Xu Mingzhu, Qin Hanjiao, Cui Shusen
Department of Hand Surgery, China Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China.
Department of Orthopedics, First Affiliated Hospital, Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China.
Neural Regen Res. 2012 Sep 15;7(26):2012-7. doi: 10.3969/j.issn.1673-5374.2012.26.002.
Studies have shown that retreatment of the distal stoma after nerve grafting can stimulate nerve regeneration. The present study attempted to verify the effects of reanastomosis of the distal stoma, after nerve grafting, on nerve regeneration by assessing brain-derived neurotrophic factor expression in 2-month-old rats. Results showed that brain-derived neurotrophic factor expression in L2-4 dorsal root ganglia began to increase 3 days after autologous nerve grafting post sciatic nerve injury, peaked at 14 days, decreased at 28 days, and reached similar levels to the sham-surgery group at 56 days. Brain-derived neurotrophic factor expression in L2-4 dorsal root ganglia began to increase 3 days after reanastomosis of the distal stoma, 59 days after autologous nerve grafting post sciatic nerve injury, significantly increased at 63 days, peaked at 70 days, and gradually decreased thereafter, but remained higher compared with the sham-surgery group up to 112 days. The results of this study indicate that reanastomosis of the distal stoma after orthotopic nerve grafting stimulated brain-derived neurotrophic factor expression in L2-4 dorsal root ganglia.
研究表明，神经移植后对远端吻合口进行再处理可刺激神经再生。本研究试图通过评估2月龄大鼠脑源性神经营养因子的表达，来验证神经移植后远端吻合口再吻合对神经再生的影响。结果显示，坐骨神经损伤后自体神经移植3天后，L2-4背根神经节中的脑源性神经营养因子表达开始增加，14天时达到峰值，28天时下降，56天时达到与假手术组相似的水平。坐骨神经损伤后自体神经移植59天后，远端吻合口再吻合3天后，L2-4背根神经节中的脑源性神经营养因子表达开始增加，63天时显著增加，70天时达到峰值，此后逐渐下降，但直至112天仍高于假手术组。本研究结果表明，原位神经移植后远端吻合口再吻合可刺激L2-4背根神经节中脑源性神经营养因子的表达。
