Seegal R F, Brosch K, Bush B, Ritz M, Shain W
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Neurotoxicology. 1989 Winter;10(4):757-64.
Pheochromocytoma (PC-12) cells synthesize, store, release and metabolize dopamine (DA) and norepinephrine (NE) in a manner analogous to that observed in the mammalian central nervous system. These cells were used to develop and validate an alternate method to animal testing to assess the effects of a complex environmental mixture of polychlorinated biphenyls (Aroclor 1254) on cellular catecholamine function. Aroclor 1254, at concentrations of 1 to 100 ppm, significantly decreased cellular catecholamine concentrations after 6 hrs. Exposure at 100 ppm for periods of less than an hr increased cellular catecholamine concentrations while longer exposure times (i.e., 1 to 24 hr) decreased cellular catecholamine concentrations. This in vitro depletion of catecholamines is similar to that seen in vivo. Thus, PC-12 cells may be useful for neurochemical evaluation of neurotoxicants with particular reference to effects on catecholaminergic systems.
嗜铬细胞瘤（PC - 12）细胞合成、储存、释放和代谢多巴胺（DA）和去甲肾上腺素（NE）的方式与哺乳动物中枢神经系统中观察到的方式类似。这些细胞被用于开发和验证一种替代动物试验的方法，以评估多氯联苯（Aroclor 1254）复杂环境混合物对细胞儿茶酚胺功能的影响。浓度为1至100 ppm的Aroclor 1254在6小时后显著降低细胞儿茶酚胺浓度。暴露于100 ppm浓度下少于1小时会增加细胞儿茶酚胺浓度，而较长的暴露时间（即1至24小时）则会降低细胞儿茶酚胺浓度。这种儿茶酚胺在体外的消耗与体内观察到的情况相似。因此，PC - 12细胞可能有助于对神经毒物进行神经化学评估，特别是对儿茶酚胺能系统的影响。
