Long Mei, Wan Xiaohui, La Xiaolin, Gong Xiaoyun, Cai Xia
Productive Center, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.
Department of Gynaecology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang Uygur Autonomous Region 830054, P.R. China.
Int J Mol Med. 2015 Apr;35(4):1119-25. doi: 10.3892/ijmm.2015.2082. Epub 2015 Jan 27.
Endometriosis is a prevalent and complex gynecological disease which affects 10% of women of reproductive age. Certain studies have suggested that a substantial number of microRNAs (miRNAs or miRs) are aberrantly or differentially expressed in the ectopic endometrium. To date, to the best of our knowledge, there is no report available on the role of miR-29 in the endometrium. In this study, we investigated the expression of the miR-29 family in the endometrium samples from women without endometriosis, as well as in paired ectopic and eutopic endometrium samples by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results revealed that miR-29c was differentially expressed in the paired eutopic and ectopic endometrium samples. In addition, c-Jun was differentially expressed in the ectopic and eutopic endometrial tissues as determined by western blot analysis. Furthermore, the role of miR-29c in endometrial cell proliferation, invasion and apoptosis was examined in vitro. The results revealed that miR-29c exerted its effects on endometrial cells by suppressing cell proliferation and invasion, as well as promoting cell apoptosis. Furthermore, it was found that c-Jun was a novel target of miR-29c, and c-Jun reversed the effects of miR-29c on the proliferation, invasion and apoptosis of endometrial cells. To the best of our knowledge, this study is the first to identify miR-29c as a suppressor of endometriosis. Taken together, our results suggest that miR-29c exerts its effects on endometrial cell proliferation, apoptosis and invasion by inhibiting the expression of c-Jun. Our data may provide a novel potential therapeutic target for the treatment of endometriosis.
子宫内膜异位症是一种常见且复杂的妇科疾病，影响10%的育龄妇女。某些研究表明，大量微小RNA（miRNA或miR）在异位子宫内膜中异常表达或差异表达。迄今为止，据我们所知，尚无关于miR-29在子宫内膜中作用的报道。在本研究中，我们通过逆转录定量聚合酶链反应（RT-qPCR）研究了miR-29家族在无子宫内膜异位症女性的子宫内膜样本以及配对的异位和在位子宫内膜样本中的表达。结果显示，miR-29c在配对的在位和异位子宫内膜样本中差异表达。此外，通过蛋白质免疫印迹分析确定，c-Jun在异位和在位子宫内膜组织中差异表达。此外，在体外检测了miR-29c在子宫内膜细胞增殖、侵袭和凋亡中的作用。结果显示，miR-29c通过抑制细胞增殖和侵袭以及促进细胞凋亡对子宫内膜细胞发挥作用。此外，发现c-Jun是miR-29c的一个新靶点，并且c-Jun逆转了miR-29c对子宫内膜细胞增殖、侵袭和凋亡的影响。据我们所知，本研究首次将miR-29c鉴定为子宫内膜异位症的抑制因子。综上所述，我们的结果表明，miR-29c通过抑制c-Jun的表达对子宫内膜细胞增殖、凋亡和侵袭发挥作用。我们的数据可能为子宫内膜异位症的治疗提供一个新的潜在治疗靶点。
