George Cindy, Smith Carine, Isaacs Ashwin W, Huisamen Barbara
Department of Biomedical Sciences, Faculty of Health Science, Stellenbosch University, Tygerberg 7505, South Africa.
Department of Physiological Sciences, Faculty of Science, Stellenbosch University, Stellenbosch 7600, South Africa.
Nutrients. 2015 Jan 23;7(2):815-30. doi: 10.3390/nu7020815.
The current treatment options for soft tissue injuries remain suboptimal and often result in delayed/incomplete recovery of damaged muscle. The current study aimed to evaluate the effects of oral Prosopis glandulosa treatment on inflammation and regeneration in skeletal muscle after contusion injury, in comparison to a conventional treatment. The gastrocnemius muscle of rats was subjected to mass-drop injury and muscle samples collected after 1-, 3 h, 1- and 7 days post-injury. Rats were treated with P. glandulosa (100 mg/kg/day) either for 8 weeks prior to injury (up until day 7 post-injury), only post-injury, or with topically applied diclofenac post-injury (0.57 mg/kg). Neutrophil (His48-positive) and macrophage (F4/80-positive) infiltration was assessed by means of immunohistochemistry. Indicators of muscle satellite cell proliferation (ADAM12) and regeneration (desmin) were used to evaluate muscle repair. Chronic P. glandulosa and diclofenac treatment (p<0.0001) was associated with suppression of the neutrophil response to contusion injury, however only chronic P. glandulosa treatment facilitated more effective muscle recovery (increased ADAM12 (p<0.05) and desmin (p<0.001) expression), while diclofenac treatment had inhibitory effects on repair, despite effective inhibition of neutrophil response. Data indicates that P. glandulosa treatment results in more effective muscle repair after contusion.
目前软组织损伤的治疗选择仍不尽人意，常常导致受损肌肉延迟恢复或恢复不完全。本研究旨在评估与传统治疗相比，口服腺牧豆树治疗对挫伤后骨骼肌炎症和再生的影响。对大鼠的腓肠肌进行重物坠落损伤，并在损伤后1小时、3小时、1天和7天采集肌肉样本。大鼠在损伤前8周（直至损伤后第7天）、仅在损伤后或在损伤后局部应用双氯芬酸（0.57mg/kg），用腺牧豆树（100mg/kg/天）进行治疗。通过免疫组织化学评估中性粒细胞（His48阳性）和巨噬细胞（F4/80阳性）浸润情况。使用肌肉卫星细胞增殖指标（ADAM12）和再生指标（结蛋白）来评估肌肉修复情况。长期使用腺牧豆树和双氯芬酸治疗（p<0.0001）与抑制中性粒细胞对挫伤损伤的反应有关，然而只有长期使用腺牧豆树治疗促进了更有效的肌肉恢复（ADAM12表达增加（p<0.05）和结蛋白表达增加（p<0.001）），而双氯芬酸治疗尽管有效抑制了中性粒细胞反应，但对修复有抑制作用。数据表明，腺牧豆树治疗可使挫伤后肌肉修复更有效。
