Research Group in Irrigated Rice, Department of Plant Science, Universidade Federal de Santa Maria (UFSM) , Santa Maria, Rio Grande do Sul, 97105-900, Brazil.
J Agric Food Chem. 2015 Mar 4;63(8):2119-26. doi: 10.1021/jf5042504. Epub 2015 Feb 23.
Thiamethoxam and chlorantraniliprole insecticides have been important tools for controlling pests in rice. However, food safety issues related to pesticide residues are important to consider with a food crop such as rice. Therefore, the objective of this study was to analyze thiamethoxam and chlorantraniliprole residues in rice hull, bran, and polished rice grains. The study was conducted during the 2012 cropping season at the Texas A&M Agrilife Research, David R. Wintermann Rice Research Station, near Eagle Lake, TX, USA. Rice was planted on May 5, 2012, using the cultivar 'Presidio'. Pesticide applications were performed at 5, 15, 25, and 35 days after flowering (DAF) using 1 and 2 times the recommended rate of 30 g active ingredient (ai) ha(-1) for thiamethoxam and 30 g ai ha(-1) for chlorantraniliprole. Sequentially, two treatments using the insecticides at recommended rate were applied at 5 and 25 DAF and at 5, 25, and 35 DAF. Insecticide residues were analyzed in different sample fractions: rice hull, bran, and polished rice grains. The samples were subjected to extraction using an accelerated solvent extraction (ASE) technique. Sample aliquots were analyzed using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), with a limit of quantification (LOQ) of 5 × 10(-5) mg kg(-1). Residues of thiamethoxam and chlorantraniliprole were detected in rice hull, bran, and polished rice grains, and the quantified values were greater in hull and in rice bran.
噻虫嗪和氯虫苯甲酰胺杀虫剂一直是防治水稻害虫的重要工具。然而，对于水稻等粮食作物，与农药残留有关的食品安全问题是需要考虑的。因此，本研究的目的是分析水稻稻壳、糠和精米中的噻虫嗪和氯虫苯甲酰胺残留量。该研究于 2012 年在美国德克萨斯州阿马里洛的德克萨斯 A&M 农业生命研究-戴维·R·温特曼水稻研究站进行，试验期间正值水稻种植季。2012 年 5 月 5 日，使用品种“Presidio”种植水稻。在开花后 5、15、25 和 35 天（DAF），使用推荐剂量的噻虫嗪 1 倍和 2 倍（30 g 有效成分（ai）ha(-1)）和氯虫苯甲酰胺 30 g ai ha(-1)）进行 1 次和 2 次施药。随后，在 5 和 25 DAF 以及 5、25 和 35 DAF 时，使用推荐剂量的杀虫剂进行了 2 次处理。采用加速溶剂萃取（ASE）技术对不同的样品部分（稻壳、糠和精米）进行提取。使用超高效液相色谱-串联质谱（UHPLC-MS/MS）分析样品等分试样，定量限（LOQ）为 5×10(-5)mg kg(-1)。在稻壳、糠和精米中检测到噻虫嗪和氯虫苯甲酰胺残留，在稻壳和糠中的定量值较高。
