Xu Mi-die, Dong Lei, Qi Peng, Weng Wei-wei, Shen Xiao-han, Ni Shu-juan, Huang Dan, Tan Cong, Sheng Wei-qi, Zhou Xiao-yan, Du Xiang
Department of Pathology, Fudan University Shanghai Cancer Center, Shanghai, 200032, China.
Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Gastric Cancer. 2016 Jan;19(1):107-15. doi: 10.1007/s10120-015-0459-2. Epub 2015 Jan 28.
Pituitary tumor-transforming gene-1 (PTTG1) is a transcription factor that can affect transcriptional activity, angiogenesis, and cell senescence. We examined PTTG1 mRNA and protein expression in gastric cancer (GC) cell lines and tissues to determine its value as a biomarker for GC diagnosis and therapy.
PTTG1 mRNA expression from 78 GC cases and paired adjacent normal mucosa (PCR cohort) as well as from five gastric cell lines was assessed using qRT-PCR. Nuclear and cytoplasmic RNA were extracted from two gastric cell lines to determine PTTG1 mRNA localization. PTTG1 protein expression from 98 GC cases, their paired adjacent normal mucosa, and 23 gastric intraepithelial neoplasia (GIN) cases was examined using immunohistochemistry (IHC cohort). The correlation between PTTG1 mRNA and protein expression and GC clinicopathological parameters was analyzed.
PTTG1 mRNA expression in GC tissues and cell lines was significantly increased compared with adjacent normal gastric mucosa and normal gastric mucous cell lines (p < 0.05). PTTG1 expression was nuclear and cytoplasmic, with higher cytoplasmic expression. PTTG1 immunostaining significantly differed in GC (95.66 ± 20.65), GIN (84.00 ± 34.16), and normal adjacent mucosa (28 ± 22.25) (p < 0.001). Multivariate Cox regression analysis revealed that PTTG1 mRNA and protein expression are independent prognostic factors for GC patient survival.
Our results suggest that PTTG1 is a promising target for GC diagnosis and therapy.
垂体肿瘤转化基因-1（PTTG1）是一种转录因子，可影响转录活性、血管生成和细胞衰老。我们检测了胃癌（GC）细胞系和组织中PTTG1 mRNA和蛋白表达，以确定其作为GC诊断和治疗生物标志物的价值。
使用qRT-PCR评估78例GC病例及其配对的相邻正常黏膜（PCR队列）以及5种胃细胞系中PTTG1 mRNA表达。从两种胃细胞系中提取核RNA和细胞质RNA，以确定PTTG1 mRNA的定位。使用免疫组织化学（IHC队列）检测98例GC病例、其配对的相邻正常黏膜以及23例胃上皮内瘤变（GIN）病例中PTTG1蛋白表达。分析PTTG1 mRNA和蛋白表达与GC临床病理参数之间的相关性。
与相邻正常胃黏膜和正常胃黏液细胞系相比，GC组织和细胞系中PTTG1 mRNA表达显著增加（p < 0.05）。PTTG1表达存在于细胞核和细胞质中，细胞质表达更高。PTTG1免疫染色在GC（95.66 ± 20.65）、GIN（84.00 ± 34.16）和相邻正常黏膜（28 ± 22.25）中存在显著差异（p < 0.001）。多变量Cox回归分析显示，PTTG1 mRNA和蛋白表达是GC患者生存的独立预后因素。
我们的结果表明，PTTG1是GC诊断和治疗的一个有前景的靶点。
