Rosa Tiago Pereira, Signoretti Fernanda Graziela Corrêa, Montagner Francisco, Gomes Brenda Paula Figueiredo de Almeida, Jacinto Rogério Castilho
Department of Restorative Dentistry, Piracicaba Dental School, Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
Department of Conservative Dentistry, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Braz Oral Res. 2015;29. doi: 10.1590/1807-3107BOR-2015.vol29.0031. Epub 2015 Jan 27.
This study investigated the presence of the Treponema species in longstanding endodontic retreatment-resistant lesions of teeth with apical periodontitis, the association of this species with clinical/radiographic features, and the association among the different target species. Microbial samples of apical lesions were collected from twenty-five adult patients referred to endodontic surgery after unsuccessful root canal retreatment. Nested-PCR and conventional PCR were used for Treponema detection. Twenty-three periradicular tissue samples showed detectable levels of bacterial DNA. Treponema species were detected in 28% (7/25) of the cases. The most frequently detected species were T. socranskii (6/25), followed by T. maltophilum (3/25), T. amylovorum (3/25), T. lecithinolyticum (3/25), T. denticola (3/25), T. pectinovorum (2/25) and T. medium (2/25). T. vicentii was not detected in any sample. Positive statistical association was found between T. socranskii and T. denticola, and between T. maltophilum and T. lecithinolyticum . No association was detected between the presence of any target microorganism and the clinical or radiographic features. Treponema spp. are present, in a low percentage, in longstanding apical lesions from teeth with endodontic retreatment failure.
本研究调查了根尖周炎患牙长期存在的根管再治疗抵抗性病变中密螺旋体属物种的存在情况、该物种与临床/影像学特征的关联以及不同目标物种之间的关联。从25例根管再治疗失败后转诊至牙髓外科的成年患者中收集根尖病变的微生物样本。采用巢式PCR和常规PCR检测密螺旋体。23份根尖周组织样本显示可检测到的细菌DNA水平。28%（7/25）的病例中检测到密螺旋体属物种。最常检测到的物种是索氏密螺旋体（6/25），其次是嗜麦芽窄食单胞菌（3/25）、解淀粉密螺旋体（3/25）、溶卵磷脂密螺旋体（3/25）、龋齿密螺旋体（3/25）、果胶分解密螺旋体（2/25）和中间密螺旋体（2/25）。在任何样本中均未检测到文森特密螺旋体。索氏密螺旋体与龋齿密螺旋体之间以及嗜麦芽窄食单胞菌与溶卵磷脂密螺旋体之间存在正相关。未检测到任何目标微生物的存在与临床或影像学特征之间存在关联。密螺旋体属物种以低比例存在于根管再治疗失败患牙的长期根尖病变中。
