Microbes Evolution Phylogeny, and Infections, Aix-Marseille Université, Institut de Recherche pour le Développement, Assistance Publique-Hopitaux de Marseille, Marseille, France.
Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
J Clin Microbiol. 2019 Sep 24;57(10). doi: 10.1128/JCM.00517-19. Print 2019 Oct.
We present here a new passive-filtration-based culture device combined with rapid identification with a new electron microscope (Hitachi TM4000) for the detection and culture of species from the human oral cavity. Of the 44 oral samples cultivated, 15 (34%) were found to be positive for using electron microscopy and were also culture positive. All were subcultured on agar plates; based on genome sequencing and analyses, 10 were strains of and 5 were strains of The 29 samples that were negative for remained culture negative. In addition, 14 species ordered from the DSMZ collection were cultured in the T-Raoult culture medium optimized here. Finally, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was used and 30 novel spectra were added to the MALDI-TOF MS database. We have successfully developed a new and effective method for treponemal detection, culture, and identification.
我们在此介绍一种新的基于被动过滤的培养装置，结合新型电子显微镜（日立 TM4000）进行快速鉴定，用于检测和培养来自人类口腔的 螺旋体。在培养的 44 个口腔样本中，15 个（34%）通过电子显微镜检测和培养呈 阳性。所有样本均在琼脂平板上进行了传代培养；根据基因组测序和分析，其中 10 个为 菌株，5 个为 菌株。29 个 阴性样本的培养结果仍为阴性。此外，在优化的 T-Raoult 培养基中培养了从 DSMZ 收藏中订购的 14 种 螺旋体。最后，采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS），并在 MALDI-TOF MS 数据库中添加了 30 个新的图谱。我们成功开发了一种新的、有效的密螺旋体检测、培养和鉴定方法。
