Tenório Thiago Ricardo dos Santos, Farah Breno Quintella, Ritti-Dias Raphael Mendes, Botero João Paulo, Brito Daniel Calado, Moura Patrícia Muniz Mendes Freire de, Prado Wagner Luiz do
Universidade de Pernambuco, Recife, PE, Brazil.
Universidade Federal de São Paulo, Santos, SP, Brazil.
Einstein (Sao Paulo). 2014 Oct-Dec;12(4):420-4. doi: 10.1590/S1679-45082014AO3214.
To compare the total and differential leukocyte count in obese and normal-weight adolescents, and to verify their possible relations with cardiorespiratory fitness and adiposity indicators.
A cross-sectional study conducted with 139 adolescents (107 obese and 32 normal weight) aged between 13 and 18 years. Cardiorespiratory fitness was determined by direct gas analysis during an incremental treadmill test. Total leukocytes and subsets were estimated by flow cytometry. Body composition was assessed by dual-energy X-ray absorptiometry. The t-test for independent samples was used for comparison between groups. The relation between leukocytes, cardiorespiratory fitness and adiposity indicators was verified by Pearson's correlation and multiple linear regression (adjusted for age and body mass index) tests.
Obese adolescents had higher leukocyte (8.12±2.36u/L x 103; p=0.001), neutrophil (4.33±1.86u/L x 103; p=0.002), and monocyte (0.70±0.22u/L x 103; p=0.002) counts compared to the levels of normal weight subjects. After the necessary adjustments, cardiorespiratory fitness had a negative association with leukocytes, neutrophils, and monocytes in boys.
Obese adolescents had higher total and differential leucocyte count when compared to normal weight individuals. We also observed a weak positive association between adiposity and total leukocyte, monocyte, and neutrophil counts, and in boys, a negative association between cardiorespiratory fitness and total count of leukocytes, monocytes, and neutrophils.
比较肥胖和正常体重青少年的白细胞总数及分类计数，并验证它们与心肺适能和肥胖指标之间的可能关系。
对139名年龄在13至18岁之间的青少年（107名肥胖者和32名正常体重者）进行了一项横断面研究。通过递增式跑步机测试期间的直接气体分析来测定心肺适能。通过流式细胞术估计白细胞总数及其亚群。采用双能X线吸收法评估身体成分。使用独立样本t检验进行组间比较。通过Pearson相关性分析和多元线性回归（根据年龄和体重指数进行调整）检验来验证白细胞、心肺适能和肥胖指标之间的关系。
与正常体重的青少年相比，肥胖青少年的白细胞（8.12±2.36u/L×10³；p=0.001）、中性粒细胞（4.33±1.86u/L×10³；p=0.002）和单核细胞（0.70±0.22u/L×10³；p=0.002）计数更高。在进行必要的调整后，心肺适能与男孩的白细胞、中性粒细胞和单核细胞呈负相关。
与正常体重个体相比，肥胖青少年的白细胞总数及分类计数更高（。我们还观察到肥胖与白细胞总数、单核细胞和中性粒细胞计数之间存在微弱的正相关，而在男孩中，心肺适能与白细胞、单核细胞和中性粒细胞的总数呈负相关。
