Zhang Peixun, Wang Zhiyong, Kou Yuhui, Han Na, Xu Chungui, Yin Xiaofeng, Wang Yanhua, Feng Xue
Department of Trauma and Orthopedics, Peking University People's Hospital Beijing, China.
Department of Anatomy and Histology, Peking University Health Science Center Beijing, China.
Am J Transl Res. 2014 Nov 22;6(6):876-85. eCollection 2014.
Among the methods of the peripheral nerve repair, artificial conduit bridging surgery is superior to epineurium and perineurium neurorrhaphy because of supplying enough space for nerve regeneration. Artificial conduit provides important microenvironment for peripheral nerve regeneration, especially for nerve amplification effect. Amplification phenomenon has been demonstrated in many studies using artificial conduit. When a finer nerve is used as a donor to connect to a distal nerve after injury, the donor nerve regenerates more lateral buds than its own fibers, which grow into distal endoneurial tubes and finally dominate the target organs. In this study, we used artificial conduit to investigate the amplification phenomenon in rats treated with Lumbricus extract as adjuvant treatment. The rats were divided into three groups at random. In the surgical groups, the proximal common peroneal nerve was used as a donor nerve to connect the distal tibial nerve. Rats in the normal group were not performed surgery. Postoperatively, the treatment group was administered Lumbricus extract as adjuvant treatment, while the model group and normal group were not given treatment. The results showed that the nerve conduction velocity, the morphometric measurements, the histological analysis and the amplification ratio in the treatment group were better than in the model group.
在周围神经修复方法中，人工导管桥接手术优于神经外膜和神经束膜缝合术，因为它为神经再生提供了足够的空间。人工导管为周围神经再生提供了重要的微环境，尤其是对神经放大效应而言。在许多使用人工导管的研究中都证实了放大现象。当使用较细的神经作为供体连接到损伤后的远端神经时，供体神经再生出的侧支芽比其自身纤维更多，这些侧支芽长入远端神经内膜管并最终支配靶器官。在本研究中，我们使用人工导管研究了地龙提取物辅助治疗大鼠的放大现象。将大鼠随机分为三组。手术组中，以腓总神经近端作为供体神经连接胫神经远端。正常组大鼠未进行手术。术后，治疗组给予地龙提取物作为辅助治疗，而模型组和正常组未给予治疗。结果显示，治疗组的神经传导速度、形态学测量、组织学分析和放大率均优于模型组。
