Chand-Bhayal Amar, Krishnaveni Devulapalli, Pandu-Ranga-Rao Kondadasula, Prabhakar Boddu, Vidyasagar Abbagani, Murali-Krishna Bal, Anita Penchikala, Jyothy Akka, Nallari Pratibha, Venkateshwari Ananthapur
Institute of Genetics and Hospital for Genetic Diseases, Osmania University, Begumpet, Hyderabad, India.
Dept. of Gastroenterology, Osmania General Hospital, Dr. NTR University of Health Sciences, Hyderabad, India.
Iran J Cancer Prev. 2012 Summer;5(3):117-23.
Gastric Cancer (GC) is one of the most commonly diagnosed malignancies. Genetic variation in genes encoding cytokines and their receptors, determine the intensity of the inflammatory response, which may contribute to individual differences in the outcome and severity of the disease. Interleukin-10 (IL-10) is a multifunctional cytokine with both immunosuppressive and antiangiogenic functions. Polymorphisms in the IL-10 gene promoter genetically determine inter-individual differences in IL-10 production. In the present study, we investigated the association between the IL-10 -1082 G/A polymorphism and the susceptibility to gastric cancer in a South Indian population from Andhra Pradesh.
We genotyped 100 patients diagnosed with gastric cancer and 132 healthy control subjects for -1082G/A single nucleotide polymorphism by Amplification Refractory Mutation System-Polymerase Chain Reaction (ARMS-PCR) method followed by agarose gel electrophoresis.
The distribution of IL-10 genotypes at -1082 G/A were GG 18 %, GA 35% and AA 47 % in gastric cancer patients and GG 31.82 %, GA 37.88 % and AA 30.3% in control subjects. The allelic frequencies of G and A were 0.355 and 0.645 in GC patients and 0.508 and 0.492 in control subjects respectively. The IL-10 -1082 A allele was associated with risk of gastric cancer (OR=1.873, 95%CI-1.285-2.73and P= 0.001048**).
Our study indicates that allele A of IL-10-1082 G/A polymorphism may be considered as one of the important risk factor in the etiology of gastric cancer.
胃癌（GC）是最常被诊断出的恶性肿瘤之一。编码细胞因子及其受体的基因中的遗传变异决定了炎症反应的强度，这可能导致疾病结局和严重程度的个体差异。白细胞介素-10（IL-10）是一种具有免疫抑制和抗血管生成功能的多功能细胞因子。IL-10基因启动子中的多态性从基因上决定了IL-10产生的个体差异。在本研究中，我们调查了印度安得拉邦的一个南印度人群中IL-10 -1082 G/A多态性与胃癌易感性之间的关联。
我们通过扩增阻滞突变系统-聚合酶链反应（ARMS-PCR）方法，随后进行琼脂糖凝胶电泳，对100例被诊断为胃癌的患者和132名健康对照者进行了-1082G/A单核苷酸多态性基因分型。
胃癌患者中IL-10 -1082 G/A基因型的分布为GG 18%、GA 35%和AA 47%，对照者中分别为GG 31.82%、GA 37.88%和AA 30.3%。GC患者中G和A的等位基因频率分别为0.355和0.645，对照者中分别为0.508和0.492。IL-10 -1082 A等位基因与胃癌风险相关（OR = 1.873，95%CI - 1.285 - 2.73，P = 0.001048**）。
我们的研究表明，IL-10 -1082 G/A多态性的A等位基因可能被视为胃癌病因中的重要危险因素之一。
