Vazirianzadeh Babak, Dehghani Rouhullah, Mehdinejad Manijeh, Sharififard Mona, Nasirabadi Nersi
Department of Medical Entomology, College of Health and Infectious and Tropical disease Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Environmental Health, College of Health and Social Determinants of Health (SDH),Research Center, Kashan University of Medical Sciences, Kashan, Iran.
J Arthropod Borne Dis. 2013 Dec 18;8(1):53-9. eCollection 2014.
The brown-banded cockroach, Supella longipalpa is known as a carrier of pathogenic bacteria in urban environments, but its role is not well documented regarding the carriage of antibiotic-resistant pathogenic bacteria in Iran. The aim of this study was to determine the resistance bacteria isolated from the brown-banded cockroach in Ahvaz, south west of Iran.
Totally 39 cockroaches were collected from kitchen area of houses and identified. All specimens were cultured to isolate the bacterial agents on blood agar and MacConky agar media. The microorganisms were identified using necessary differential and biochemical tests. Antimicrobial susceptibility tests were performed for isolated organisms by Kirby-Bauer's disk diffusion according to NCLI guideline, using 18 antibiotics.
From the 39 collected S. langipalpa, 179 bacterial agents were isolated, 92 of alimentary ducts and 87 of external body surfaces. Isolated bacteria from cockroaches were identified as Enterobacter spp., Klebsiella spp., Citrobacter spp., Escherichia coli, Salmonella spp., Proteus spp., coagulase negative staphylococci, Serratia marcescens, Staphylococcus aureus, and Bacillus species. The pattern resistance rates were determined for gram negative bacilli and gram positive cocci regarding 18 antibiotics.
The brown-banded cockroach can be involved in the spread of drug resistant bacteria and increases the possibility of contacting human environment to drug resistant bacteria. Therefore, the potential of removing this insect should be improved. This is the first original report of drug resistant bacteria isolated from the brown-banded cockroach of Iran.
褐斑大蠊（Supella longipalpa）在城市环境中被认为是病原菌的携带者，但在伊朗，其在携带耐抗生素病原菌方面的作用尚未得到充分记录。本研究的目的是确定从伊朗西南部阿瓦士的褐斑大蠊中分离出的耐药菌。
从房屋厨房区域共收集39只蟑螂并进行鉴定。所有标本在血琼脂和麦康凯琼脂培养基上培养以分离细菌病原体。使用必要的鉴别和生化试验鉴定微生物。根据NCLI指南，采用18种抗生素，通过 Kirby-Bauer 纸片扩散法对分离出的菌株进行药敏试验。
从39只收集到的褐斑大蠊中，分离出179株细菌病原体，其中92株来自消化道，87株来自体表。从蟑螂中分离出的细菌被鉴定为肠杆菌属、克雷伯菌属、柠檬酸杆菌属、大肠杆菌、沙门氏菌属、变形杆菌属、凝固酶阴性葡萄球菌、粘质沙雷氏菌、金黄色葡萄球菌和芽孢杆菌属。测定了革兰氏阴性杆菌和革兰氏阳性球菌对18种抗生素的耐药率模式。
褐斑大蠊可能参与耐药菌的传播，并增加人类环境接触耐药菌的可能性。因此，应提高清除这种昆虫的能力。这是首次从伊朗褐斑大蠊中分离出耐药菌的原始报告。
