Li Chao, Wang Zheng-Liang, Fang Wen-Yuan, Yu Xiao-Ping
a Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine , College of Life Sciences, China Jiliang University , Hangzhou , Zhejiang , People's Republic of China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4035-4036. doi: 10.3109/19401736.2014.1003831. Epub 2015 Jan 28.
The complete mitochondrial genome of an orb-weaving spider Neoscona theisi (Walckenaer) is determined in this article. It is a typical circular duplex DNA molecule with a length of 14,156 bp, which encodes the same 37 genes as all metazoan mitogenomes and an A + T-rich region (D-loop). The overall A + T content is 75.2% (A: 35.5%; T: 39.8%; G: 15.6%; C: 9.3%). All of the protein-coding genes were initiated by ATN, with the exceptions in three genes. COI has a TTA start codon, COII, COIII and ND6 use TTG as initiation codon. Three genes (ND3, COII and ND5) end with incomplete stop codons (T or TA), while all other genes terminated with canonical stop codons (TAA or TAG). Among 22 transfer RNAs genes, six of them (tRNA, tRNA, tRNA, tRNA, tRNA and tRNA) lack the potential to form the cloverleaf-shaped secondary structure. The A + T-rich region is 559 bp with an A + T content of 79.6%.
本文测定了圆蛛科蜘蛛大腹园蛛（Neoscona theisi (Walckenaer)）的完整线粒体基因组。它是一个典型的环状双链DNA分子，长度为14,156 bp，编码与所有后生动物线粒体基因组相同的37个基因以及一个富含A+T的区域（D环）。总的A+T含量为75.2%（A：35.5%；T：39.8%；G：15.6%；C：9.3%）。所有蛋白质编码基因均以ATN起始，但有三个基因例外。COI以TTA作为起始密码子，COII、COIII和ND6以TTG作为起始密码子。三个基因（ND3、COII和ND5）以不完整的终止密码子（T或TA）结束，而所有其他基因以典型的终止密码子（TAA或TAG）结束。在22个转运RNA基因中，有六个（tRNA、tRNA、tRNA、tRNA、tRNA和tRNA）缺乏形成三叶形二级结构的潜力。富含A+T的区域为559 bp，A+T含量为79.6%。
