Guan De-Long, Xu Sheng-Quan
a Co-Innovation Center for Qinba Regions' Sustainable Development , College of Life Sciences, Shaanxi Normal University , Xi'an , Shaanxi , PR China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4052-4053. doi: 10.3109/19401736.2014.1003839. Epub 2015 Jan 28.
Based on whole-genome Illumina sequencing data, the complete mitogenome of the Asiatic Salamander Batrachuperus tibetanus was reconstructed and characterized. The circular genome is 16,375 bp in length, and typically consists of 37 genes, including 13 protein coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs) and 1 D-loop region. Coding genes (PCGs, tRNAs & rRNAs) pieces is 15,460 bp in length, accounting for 94.41% of the total length of genome. The nucleotide composition is asymmetric (34.1% A, 19.5% C, 13.7% G, 32.7% T) with an overall GC content of 33.2%. Comparing our research data with predecessors' previous work, significant changes was found with 94.0% of whole genome sequence consistency percentage (Pairwise % Identity). We suggest our data would contribute to the evolutionary studies of this and related Asiatic Salamanders species.
基于全基因组Illumina测序数据,重建并表征了西藏山溪鲵的完整线粒体基因组。该环状基因组长度为16,375 bp,通常由37个基因组成,包括13个蛋白质编码基因(PCGs)、22个转运RNA(tRNAs)、2个核糖体RNA(rRNAs)和1个D-loop区域。编码基因(PCGs、tRNAs和rRNAs)片段长度为15,460 bp,占基因组总长度的94.41%。核苷酸组成不对称(A占34.1%,C占19.5%,G占13.7%,T占32.7%),总体GC含量为33.2%。将我们的研究数据与前人的工作进行比较,发现有显著变化,全基因组序列一致性百分比(成对%同一性)为94.0%。我们认为我们的数据将有助于对该物种及相关亚洲蝾螈物种的进化研究。