嘌呤核苷单磷酸中羟基诱导的自由基：电子自旋共振和自旋捕获
OH-induced free radicals in purine nucleoside monophosphates: e.s.r. and spin-trapping.
作者信息
Hiraoka W, Kuwabara M, Sato F
机构信息
Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
出版信息
Int J Radiat Biol. 1989 Jan;55(1):51-8. doi: 10.1080/09553008914550061.
Free radicals produced by the reactions of OH radicals with six purine nucleoside monophosphates (3'-AMP, 5'-AMP, 5'-dAMP, 3'-GMP, 5'-GMP and 5'-dGMP) were investigated by a method combining e.s.r. spin-trapping and high-performance liquid chromatography (HPLC). The N2O-saturated aqueous solutions of purine nucleoside monophosphates, containing 2-methyl-2-nitrosopropane as a spin-trap, were X-irradiated and the resulting spin-adducts were separated by reverse-phase HPLC in the ion suppression mode. The separated spin-adducts were characterized by e.s.r. spectrometry and UV spectrophotometry. Consequently, the radicals due to H-abstraction at the C4' position of the sugar moiety were identified arising from 5'-dAMP and 5'-dGMP. In all cases, e.s.r. spectra consisting of a secondary doublet were observed and assigned to the radical due to H-abstraction at the C5' position of the sugar moiety.
采用电子自旋共振（e.s.r.）自旋捕集和高效液相色谱（HPLC）相结合的方法，研究了羟基自由基（OH自由基）与六种嘌呤核苷单磷酸（3'-AMP、5'-AMP、5'-dAMP、3'-GMP、5'-GMP和5'-dGMP）反应产生的自由基。将含有2-甲基-2-亚硝基丙烷作为自旋捕集剂的嘌呤核苷单磷酸的N2O饱和水溶液进行X射线辐照，然后在离子抑制模式下通过反相HPLC分离得到的自旋加合物。通过电子自旋共振光谱和紫外分光光度法对分离得到的自旋加合物进行表征。结果表明，5'-dAMP和5'-dGMP会在糖部分的C4'位置发生氢原子提取产生自由基。在所有情况下，均观察到由二级双峰组成的电子自旋共振光谱，并将其归因于糖部分C5'位置的氢原子提取产生的自由基。
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