Nanjing Drum Tower Hospital, The Affiliated Hospital of Nanjing University Medical School , Nanjing 210008, China.
ACS Appl Mater Interfaces. 2015 Feb 25;7(7):4401-5. doi: 10.1021/am5089378. Epub 2015 Feb 10.
In this work, a surface-confined peptide network that can exhibit distinct structural features under protease cleavage and electrochemical treatment is developed as a highly sensitive biosensor for clinical detection of kallikrein 6 (KLK6). KLK6 is a serine protease of the tissue-remodeling process determining the development of hepatocellular carcinoma (HCC). The peptide network, immobilized on the electrode surface by a Au-S bond, loses its integrity upon KLK6 cleavage and is removed from the electrode by electrochemical desorption of thiol groups, while the network without protease cleavage can remain attached by a few intact Au-S bonds. In this manner, distinct conductivity of the electrode surface in the presence/absence of protease can result in a large signal-to-background ratio, enabling KLK6 detection in clinical samples. The detected KLK6 abundance can manifest the correlation between up-regulated KLK6 activity and the progress of HCC. These results suggest a potential future use of this peptide network as a biosensor to provide diagnostic information for better administration of HCC.
在这项工作中，开发了一种表面限制的肽网络，该网络在蛋白酶切割和电化学处理下可以表现出明显的结构特征，可作为用于临床检测激肽释放酶 6（KLK6）的高灵敏度生物传感器。KLK6 是一种丝氨酸蛋白酶，参与组织重塑过程，决定了肝细胞癌（HCC）的发展。肽网络通过 Au-S 键固定在电极表面上，在 KLK6 切割时会失去完整性，并通过硫醇基团的电化学解吸从电极上除去，而没有蛋白酶切割的网络可以通过几个完整的 Au-S 键保持附着。通过这种方式，存在/不存在蛋白酶时电极表面的明显导电性会导致大的信号背景比，从而能够在临床样本中检测 KLK6。检测到的 KLK6 丰度可以表现出上调的 KLK6 活性与 HCC 进展之间的相关性。这些结果表明，这种肽网络作为生物传感器具有潜在的未来用途，可以为 HCC 的更好管理提供诊断信息。
