del Moral Zamora Beatriz, Álvarez Azpeitia Juan Manuel, Oliva Brañas Ana Maria, Colomer-Farrarons Jordi, Castellarnau Marc, Miribel-Català Pere Ll, Homs-Corbera Antoni, Juárez Antonio, Samitier Josep
Department of Electronics, Discrete to Integrated Electronics (D2In) group, University of Barcelona, Barcelona, Spain.
Nanobioengineering group, Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
Electrophoresis. 2015 Jul;36(13):1405-13. doi: 10.1002/elps.201400433. Epub 2015 Apr 14.
We describe a novel continuous-flow cell concentrator microdevice based on dielectrophoresis, and its associated custom-made control unit. The performances of a classical interdigitated metal electrode-based dielectrophoresis microfluidic device and this enhanced version, that includes insulator-based pole structures, were compared using the same setup. Escherichia coli samples were concentrated at several continuous flows and the device's trapping efficiencies were evaluated by exhaustive cell counts. Our results show that pole structures enhance the retention up to 12.6%, obtaining significant differences for flow rates up to 20 μL/min, when compared to an equivalent classical interdigitated electrodes setup. In addition, we performed a subsequent proteomic analysis to evaluate the viability of the biological samples after the long exposure to the actuating electrical field. No Escherichia coli protein alteration in any of the two systems was observed.
我们描述了一种基于介电泳的新型连续流细胞浓缩微器件及其相关的定制控制单元。使用相同的装置比较了基于经典叉指状金属电极的介电泳微流控装置和这种增强版本（包括基于绝缘体的极结构）的性能。在几个连续流中对大肠杆菌样本进行浓缩，并通过详尽的细胞计数评估该装置的捕获效率。我们的结果表明，与等效的经典叉指电极装置相比，极结构可将保留率提高至12.6%，在流速高达20 μL/min时获得显著差异。此外，我们进行了后续的蛋白质组学分析，以评估生物样本在长时间暴露于驱动电场后的活力。在两个系统中的任何一个中均未观察到大肠杆菌蛋白质的改变。
