Yang Rui, Guan De-Long, Xu Sheng-Quan
a College of Life Sciences, Shaanxi Normal University , Xi'an , Shaanxi , P. R. China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3240-1. doi: 10.3109/19401736.2015.1007364. Epub 2015 Jan 29.
The whole-genome Illumina sequence of the Chinese endemic grasshopper Fruhstorferiola kulinga mitogenome was constructed and reported in this study. In all, the circular genome was obtained with 15,655 bp in length and contains 75.4% A + T. It typically consists of 37 genes, including 13 protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), 2 ribosomal RNAs (rRNAs) and 1 D-loop region. All PCGs are initiated with ATN codons. Most of the PCGs use TAA as their stop codons, while the others use TAG as stop codons (COX1 and ND1). The size of the large and small ribosomal RNA genes are 1314 bp and 851 bp. The A + T-rich region (777 bp) showed strong resemblance to the other known Orthoptera insects. Our data would contribute to confirm the close relationship and other evolutionary researches of the F. kulinga.
本研究构建并报道了中国特有蝗虫库氏佛蝗线粒体基因组的全基因组Illumina序列。总体而言，获得的环状基因组长度为15,655 bp，A+T含量为75.4%。它通常由37个基因组成，包括13个蛋白质编码基因（PCGs）、22个转运RNA（tRNAs）、2个核糖体RNA（rRNAs）和1个D环区域。所有PCGs均以ATN密码子起始。大多数PCGs使用TAA作为其终止密码子，而其他的则使用TAG作为终止密码子（COX1和ND1）。大、小核糖体RNA基因的大小分别为1314 bp和851 bp。富含A+T的区域（777 bp）与其他已知直翅目昆虫有很强的相似性。我们的数据将有助于证实库氏佛蝗的密切关系和其他进化研究。
