Zhang Chulin, Mao Benyong, Wang Hanqiang, Dai Li, Huang Yuan, Chen Zhilin, Huang Jianhua
Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees (Central South University of Forestry and Technology), Ministry of Education, Changsha 410004, China.
Guangxi Key Laboratory of Rare and Endangered Animal Ecology, Guangxi Normal University, Guilin 541004, China.
Insects. 2023 Jan 13;14(1):85. doi: 10.3390/insects14010085.
Clarifying phylogenetic position and reconstructing robust phylogeny of groups using various evidences are an eternal theme for taxonomy and systematics. In this study, the complete mitogenomes of Longzhouacris mirabilis, Ranacris albicornis, and Conophyma zhaosuensis were sequenced using next-generation sequencing (NGS), and the characteristics of the mitogenomes are presented briefly. The mitogenomes of the three species are all circular molecules with total lengths of 16,164 bp, 15,720 bp, and 16,190 bp, respectively. The gene structures and orders, as well as the characteristics of the mitogenomes, are similar to those of other published mitogenomes in Caelifera. The phylogeny of the main subfamilies of Acrididae with prosternal process was reconstructed using a selected dataset of mitogenome sequences under maximum likelihood (ML) and Bayesian inference (BI) frameworks. The results showed that the genus Emeiacris consistently fell into the subfamily Melanoplinae rather than Oxyinae, and the genus Choroedocus had the closest relationship with Shirackiacris of the subfamily Eyprepocnemidinae in both phylogenetic trees deduced from mitogenome protein coding genes (PCGs). This finding is entirely consistent with the morphological characters, which indicate that Emeiacris belongs to Melanoplinae and Choroedocus belongs to Eyprepocnemidinae. In addition, the genera Conophymacris and Xiangelilacris, as well as Ranacris and Menglacris, are two pairs of the closest relatives, but their phylogenetic positions need further study to clarify.
利用各种证据阐明系统发育位置并重建群体的稳健系统发育是分类学和系统学的永恒主题。在本研究中，使用下一代测序（NGS）对奇异隆州蝗、白角蛙蝗和昭苏皱腹蝗的完整线粒体基因组进行了测序，并简要介绍了线粒体基因组的特征。这三个物种的线粒体基因组均为环状分子，全长分别为16,164 bp、15,720 bp和16,190 bp。基因结构和顺序以及线粒体基因组的特征与其他已发表的蝗亚目线粒体基因组相似。利用线粒体基因组序列的选定数据集，在最大似然（ML）和贝叶斯推断（BI）框架下重建了具有前胸背板突起的蝗科主要亚科的系统发育。结果表明，在由线粒体基因组蛋白质编码基因（PCGs）推导的两个系统发育树中，峨眉蝗属始终归入黑蝗亚科而非尖翅蝗亚科，且柯氏蝗属与皱腹蝗亚科的史氏蝗属关系最为密切。这一发现与形态特征完全一致，表明峨眉蝗属属于黑蝗亚科，柯氏蝗属属于皱腹蝗亚科。此外，皱腹蝗属和湘蝗属以及蛙蝗属和勐腊蝗属是两对亲缘关系最近的属，但它们的系统发育位置需要进一步研究以明确。
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