Liu Xueqing, Du Hejun, Wu Chuan, Xiao Kan, Qu Huantao, Zhang Xu, Gao Yong, Chen Lei
a Institute of Chinese Sturgeon, China Three Gorges Corporation , Yichang , China and.
b Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes , Institute of Chinese Sturgeon, China Three Gorges Corporation , Yichang , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3242-3. doi: 10.3109/19401736.2015.1007365. Epub 2015 Jan 29.
The complete mitochondrial genome of Ictiobus cyprinellus was first determined by a polymerase chain reaction-based sequencing method in this study. The mitochondria was 16,611 bp in length, including 13 protein genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and 1 control region. All genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The gene arrangement of I. cyprinellus was similar to those found in the other Catostomidae species. Base composition of the heavy strain was A (29.40%), T (24.86%), C (27.96%), G (17.78%) and with A + T bias of 54.26%.
本研究首次采用基于聚合酶链反应的测序方法测定了大眼牛胭脂鱼的完整线粒体基因组。线粒体长度为16,611 bp，包括13个蛋白质基因、2个核糖体RNA（rRNA）基因、22个转运RNA（tRNA）基因和1个控制区。除ND6和8个tRNA基因外，所有基因均在重链上编码。大眼牛胭脂鱼的基因排列与其他胭脂鱼科物种相似。重链的碱基组成为A（29.40%）、T（24.86%）、C（27.96%）、G（17.78%），A+T偏倚率为54.26%。
