Liu Chang Zhong, Wei Guang Hui, Hu Jian He, Liu Xing You
a College of Animal Science, Henan Institute of Science and Technology , Xinxiang , Henan Province , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Jul;27(4):2421-2. doi: 10.3109/19401736.2015.1030624. Epub 2015 Apr 29.
In this study, the complete mitochondrial genome sequence of the Paracobitis variegates was first reported. The total length of the mitogenome is 16,571 bp long with the A + T content of 55.6%. It contains the typical structure, including 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and one D-loop region. The protein-coding genes start with the typical ATG codon, while COI gene uses GTG as the initiation codon. Most tRNA genes could form typical secondary structures except tRNA(ser), which had an absence of the DHU arm. There are 43 helices structures in 12S rRNA, and six domains, 53 helices structures in 16S rRNA. According to the phylogenetic analysis, Paracobitis variegates has a closer relationship with Barbatula toni.
本研究首次报道了杂斑副鳅的完整线粒体基因组序列。该线粒体基因组全长16,571 bp，A+T含量为55.6%。它具有典型的结构，包括13个蛋白质编码基因、22个转运RNA基因、两个核糖体RNA基因和一个D环区域。蛋白质编码基因以典型的ATG密码子起始，而COI基因使用GTG作为起始密码子。除了缺少DHU臂的tRNA(ser)外，大多数tRNA基因可以形成典型的二级结构。12S rRNA中有43个螺旋结构，16S rRNA中有六个结构域、53个螺旋结构。根据系统发育分析，杂斑副鳅与托尼高原鳅关系更近。
