Zhang Yanfang, Xie Zhixun, Deng Xianwen, Xie Zhiqin, Liu Jiabo, Xie Liji, Luo Sisi, Huang Li, Huang Jiaoling, Zeng Tingting, Wang Sheng
a Department of Biotechnology , Guangxi Key Laboratory of Animal Vaccines and Diagnostics, Guangxi Veterinary Research Institute , Nanning , Guangxi , PR China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4139-4141. doi: 10.3109/19401736.2014.1003881. Epub 2015 Jan 29.
The complete mitochondrial genome sequence of the Luchuan pig was measured by PCR-based methods, primer-walking sequencing, and fragment cloning. The entire mitochondrial genome of the Luchuan pig was identified as a circular molecule consisting of 16,730 bp (GenBank accession number: KP126954). The contents of A, T, C, and G in the mitochondrial genome were found to be 34.65%, 25.80%, 26.19%, and 13.36%, respectively. The complete mitochondrial genome of the Luchuan pig contains a typical structure, including 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and one control region (D-loop region). This complete mitochondrial genome sequence provides essential information in understanding phylogenetic relationships among Sus scrofa domestic mitochondrial genomes.
采用基于聚合酶链式反应（PCR）的方法、引物步移测序法和片段克隆法测定了陆川猪的完整线粒体基因组序列。陆川猪的整个线粒体基因组被鉴定为一个由16730 bp组成的环状分子（GenBank登录号：KP126954）。发现线粒体基因组中A、T、C和G的含量分别为34.65%、25.80%、26.19%和13.36%。陆川猪的完整线粒体基因组具有典型结构，包括13个蛋白质编码基因、两个核糖体RNA基因、22个转运RNA基因和一个控制区（D环区）。该完整线粒体基因组序列为理解家猪线粒体基因组之间的系统发育关系提供了重要信息。
