Bros Helena, Niesner Raluca, Infante-Duarte Carmen
Institute for Medical Immunology, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany,
Methods Mol Biol. 2015;1264:465-72. doi: 10.1007/978-1-4939-2257-4_38.
Distribution of mitochondria throughout the cytoplasm is necessary for cellular function and health. Due to their unique, highly polarized morphology, neurons are particularly vulnerable to defects of mitochondrial transport, and its disruption can contribute to neuropathology. In this chapter, we present an ex vivo method for monitoring mitochondrial transport within myelinated sensory and motor axons from spinal nerve roots. This approach can be used to investigate mitochondrial behavior under a number of experimental conditions, e.g., by applying ion channel modulators, ionophores, or toxins, as well as for testing the therapeutic potential of new strategies targeting axonal mitochondrial dynamics.
线粒体在整个细胞质中的分布对于细胞功能和健康至关重要。由于其独特的、高度极化的形态，神经元特别容易受到线粒体运输缺陷的影响，而这种运输的破坏会导致神经病理学。在本章中，我们介绍一种用于监测来自脊神经根的有髓感觉和运动轴突内线粒体运输的离体方法。这种方法可用于在多种实验条件下研究线粒体行为，例如通过应用离子通道调节剂、离子载体或毒素，以及用于测试针对轴突线粒体动力学的新策略的治疗潜力。
