State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan; Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan.
Bioresour Technol. 2015 Mar;180:381-5. doi: 10.1016/j.biortech.2015.01.030. Epub 2015 Jan 16.
Pseudomonas sp. C27 can effectively conduct mixotrophic denitrifying sulfide removal (DSR) reactions using both organic matters and sulfide as electron donors. This study conducted DSR tests using C27 and quantitatively analyzed the protein abundances at C/N=1.26, 1.63 and 3.0. At C/N=1.26, C27 principally adopted autotrophic denitrification pathway in DSR reaction. As C/N ratio was increased to 1.63, C27 enhanced heterotrophic denitrification pathway for removing nitrous compounds. As the C/N ratio was further increased to 3.0, C27 accelerated metabolism via coupled-cycles pathway. The C/N ratio for coupled-cycles pathway was estimated ranging 2.0-2.3 in the studied medium. Optimal C/N ratio of traditional DSR processes ranged 1.05-1.26. With the coupled-cycles pathway, the accessible C/N/S regime for C27 on DSR reactions is enlarged. Minor revision of the coupled-cycles pathway considering production of ammonium step was made.
铜绿假单胞菌 C27 可以有效地利用有机物和硫化物作为电子供体进行混合营养型反硝化脱硫（DSR）反应。本研究使用 C27 进行了 DSR 测试，并在 C/N=1.26、1.63 和 3.0 时定量分析了蛋白质丰度。在 C/N=1.26 时，C27 在 DSR 反应中主要采用自养反硝化途径。随着 C/N 比增加到 1.63，C27 增强了异养反硝化途径以去除亚硝化合物。当 C/N 比进一步增加到 3.0 时，C27 通过偶联循环途径加速了代谢。在研究的培养基中，偶联循环途径的 C/N 比估计在 2.0-2.3 之间。传统 DSR 工艺的最佳 C/N 比范围为 1.05-1.26。通过偶联循环途径，C27 在 DSR 反应中可利用的 C/N/S 范围扩大了。考虑到氨的产生步骤，对偶联循环途径进行了微小的修正。
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