Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan; Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan.
Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan.
Bioresour Technol. 2014 Aug;166:616-9. doi: 10.1016/j.biortech.2014.05.099. Epub 2014 Jun 2.
Denitrifying sulfide removal (DSR) process simultaneously removes nitrate, sulfide and organic matters in the same reactor. This study applied Azoarcus sp. NSC3 and Pseudomonas sp. CRS1 mix for DSR tests in autotrophic, heterotrophic and mixotrophic growths. Negligible NO-compounds were noted in heterotrophic or mixotrophic growths, while most cells were damaged and bound with NO-compounds in autotrophic growth. Nitroprusside (SNP) ions were applied as model compound to reveal the formation of nitrososulfide complex (RSNO) by nitroso (NO(+)) and excess sulfide (S(2-)), rather than the previously proposed mechanism by direct reaction between nitric oxide (NO) and S(2-). We speculated that RSNO was then abiotically decomposed to NO and elemental sulfur in the presence of biological cells. A revised nitrogen cycle considering interactions with sulfur compounds was proposed. We also speculated that SNO and NO were inhibitory to the functional strains, whose efficient removals were essential to reach high-rate DSR performance.
反硝化硫去除（DSR）过程在同一个反应器中同时去除硝酸盐、硫化物和有机物。本研究应用 Azoarcus sp. NSC3 和 Pseudomonas sp. CRS1 混合物进行自养、异养和混合生长的 DSR 测试。在异养或混合生长中几乎没有检测到 NO 化合物，而在自养生长中，大多数细胞受损并与 NO 化合物结合。硝普盐（SNP）离子被用作模型化合物，以揭示亚硝基（NO(+)) 和过量硫化物（S(2-))之间形成亚硝酰硫复合物（RSNO），而不是之前提出的一氧化氮（NO）和 S(2-)之间直接反应的机制。我们推测，在生物细胞存在的情况下，RSNO 会被非生物分解为 NO 和元素硫。提出了一个考虑与硫化合物相互作用的修订氮循环。我们还推测 SNO 和 NO 对功能菌株具有抑制作用，高效去除这些菌株对于达到高 DSR 性能至关重要。
