Biliński T, Litwińska J, Błaszczyński M, Bajus A
Zamosc College of Agriculture, Agricultural Academy of Lublin, Poland.
Biochim Biophys Acta. 1989 Jan 23;1001(1):102-6. doi: 10.1016/0005-2760(89)90312-3.
Mutants of Saccharomyces cerevisiae, deficient in cytosolic superoxide dismutase and catalase activities were used to study the role of various oxygen species in the process of lipid peroxidation in yeast cells. Lipid peroxidation does not occur normally in yeast, because this organism is unable to form fatty acids with more than one double bond, whereas under physiological conditions, only fatty acids with at least two double bonds undergo this process. The fatty acid content of cellular lipids was modified by growing the cells in anoxia in the presence of oleic or linolenic acid. Toxic effects of oxygen were observed almost exclusively in those cells of yeast mutants deficient in superoxide dismutase, which contain linolenic acid in cellular lipids. Hypersensitivity of the mutant cells, however, results mainly from toxic effects of the products of autooxidation of extracellular fatty acids. These facts suggest that superoxide dismutases are in some way involved in preventing toxic effects of the products of lipid peroxidation and to some extent prevent the process of lipid peroxidation.
利用缺乏胞质超氧化物歧化酶和过氧化氢酶活性的酿酒酵母突变体，研究各种氧物种在酵母细胞脂质过氧化过程中的作用。脂质过氧化在酵母中通常不会发生，因为这种生物体无法形成具有多个双键的脂肪酸，而在生理条件下，只有具有至少两个双键的脂肪酸才会经历这个过程。通过在缺氧条件下在油酸或亚麻酸存在下培养细胞，改变细胞脂质中的脂肪酸含量。几乎仅在缺乏超氧化物歧化酶的酵母突变体细胞中观察到氧的毒性作用，这些细胞的细胞脂质中含有亚麻酸。然而，突变体细胞的超敏反应主要源于细胞外脂肪酸自氧化产物的毒性作用。这些事实表明，超氧化物歧化酶在某种程度上参与预防脂质过氧化产物的毒性作用，并在一定程度上防止脂质过氧化过程。
