Khajehnasiri Farahnaz, Akhondzadeh Shahin, Mortazavi Seyed Bagher, Allameh Abdolamir, Khavanin Ali, Zamanian Zahra
Department of Social Medicine, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Psychiatry and Psychology Research Center, Roozbeh Hospital, Tehran University of Medical Sciences. Tehran, Iran.
Iran J Psychiatry. 2014 Apr;9(2):76-82.
The aim of this study was to determine the oxidative stress; serum level of Total Antioxidant Capacity (TAC)and Malondialdehyde (MDA) level and the depression score among the depressed rotational shift workers in Shahid Tondgouyan Refinery in Tehran (Iran).
A cross-sectional study was conducted among all the 189 shift workers in Shahid Tondgouyan oil refinery who were eligible to participate in the study. They did not take any antidepressants for two months or any supplements for two weeks prior to the study entry. Written consent was obtained from the participants. 21- Item Beck Depression Inventory was used to measure the depression level. Furthermore, body weight, height and systolic and diastolic blood pressure were collected from all the participants. The levels of Total Antioxidant Capacity (TAC) and Malondialdehyde (MDA) were measured by 8 ml fasting blood sample. MDA was determined by thiobarbituric acid reaction. Serum total antioxidants were measured using the spectrophotometric ABTS. In the ABTS test, 2,2'-azinobis (3-ethylbenzthiazoline-6-acid) (ABTS) is converted into its radical cation (ABTS•+) by addition of sodium persulphate. This blue-green radical cation absorbs light at 734 nm. ABTS•+ is reactive towards most antioxidants. Descriptive statistics, ANOVA, ANCOVA and regression tests and correlation were used to analyze the data using SPSS software version 16.
The age of the participants ranged from 21 to 52 years. The mean age of the participants was 30.58 year (±6.97yr). Of all the participants, 28% (n= 53) had no depression symptoms (depression score between 0 and 9), 65.1% (n=123) were categorized as having mild depression (depression score between 10 and 18) and 6.9% (n=13) were categorized as having moderate depression (depression score between 19 and 29). The participants' BMI ranged from 15.9 to 34.3; the mean BMI of the participants was 24.82 kg/m(2) (+ 3.81 kg/m(2)). The mean of the serum TAC level was 2.51 (± 0.56) mg/dl, and the mean serum MDA level was 3.67(± 1.08) μmol/l. There was a significant difference in the mean TAC concentration between the non-depressed group and the group with mild depression (p=0.029).
Depression was associated with reduced mean TAC concentration and an increase in MDA level. There was a linear relationship between the depression score and shift work experience among the rotational shift workers, which showed a high level of stress and depression among the shift-workers.
本研究旨在确定德黑兰（伊朗）沙希德·通德古扬炼油厂抑郁倒班工人的氧化应激、血清总抗氧化能力（TAC）水平、丙二醛（MDA）水平及抑郁评分。
对沙希德·通德古扬炼油厂所有符合参与研究条件的189名倒班工人进行了一项横断面研究。在研究开始前两个月，他们未服用任何抗抑郁药，两周内未服用任何补充剂。获得了参与者的书面同意。使用21项贝克抑郁量表来测量抑郁水平。此外，收集了所有参与者的体重、身高以及收缩压和舒张压。通过8毫升空腹血样测量总抗氧化能力（TAC）和丙二醛（MDA）的水平。MDA通过硫代巴比妥酸反应测定。血清总抗氧化剂使用分光光度法ABTS进行测量。在ABTS测试中，通过添加过硫酸钠将2,2'-联氮双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）转化为其自由基阳离子（ABTS•+）。这种蓝绿色自由基阳离子在734纳米处吸收光。ABTS•+对大多数抗氧化剂具有反应性。使用SPSS软件16版进行描述性统计、方差分析、协方差分析、回归测试和相关性分析来分析数据。
参与者的年龄在21至52岁之间。参与者的平均年龄为30.58岁（±6.97岁）。在所有参与者中，28%（n = 53）没有抑郁症状（抑郁评分在0至9之间），65.1%（n = 123）被归类为轻度抑郁（抑郁评分在10至18之间），6.9%（n = 13）被归类为中度抑郁（抑郁评分在19至29之间）。参与者的BMI范围为15.9至34.3；参与者的平均BMI为24.82 kg/m²（±3.81 kg/m²）。血清TAC水平的平均值为2.51（±0.56）mg/dl，血清MDA水平的平均值为3.67（±1.08）μmol/l。非抑郁组和轻度抑郁组之间的平均TAC浓度存在显著差异（p = 0.029）。
抑郁与平均TAC浓度降低和MDA水平升高有关。倒班工人的抑郁评分与倒班工作经历之间存在线性关系，这表明倒班工人中存在高度的压力和抑郁。
