提高产油海洋硅藻日光拟菱形藻JPCC DA0580中甘油代谢以提高三酰甘油生产力。
Enhancement of glycerol metabolism in the oleaginous marine diatom Fistulifera solaris JPCC DA0580 to improve triacylglycerol productivity.
作者信息
Muto Masaki, Tanaka Masayoshi, Liang Yue, Yoshino Tomoko, Matsumoto Mitsufumi, Tanaka Tsuyoshi
机构信息
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588 Japan ; JST, CREST, Sanbancho 5, Chiyoda-ku, Tokyo 102-0075 Japan.
Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei, Tokyo 184-8588 Japan.
出版信息
Biotechnol Biofuels. 2015 Jan 22;8(1):4. doi: 10.1186/s13068-014-0184-9. eCollection 2015.
BACKGROUND
Microalgal oil is a promising alternative feedstock for biodiesel fuel (BDF). Mixotrophic cultivation with glycerol, the primary byproduct of BDF production, may be used to optimize BDF production. This strategy would reduce costs through glycerol recycling and improve lipid productivity and biomass productivity by overcoming the growth retardation caused by decreased light penetration in high-density culture.
RESULTS
Overexpression of the endogenous glycerol kinase (GK) gene in an oleaginous marine diatom, Fistulifera solaris JPCC DA0580, accelerates glycerol metabolism and improves lipid and biomass productivities. Two candidates were selected from a collection of 90 G418-resistant clones, based on growth and confirmation of genome integration. GK gene expression was higher in the selected clones (GK1_7 and GK2_16) than in the wild-type culture. The GK2_16 clone achieved a 12% increase in lipid productivity.
CONCLUSION
We have demonstrated the potential of metabolic engineering in oleaginous microalgae to improve lipid productivity. Metabolic engineering techniques can be used to optimize BDF production.
背景
微藻油是一种很有前景的生物柴油燃料（BDF）替代原料。利用甘油（BDF生产的主要副产物）进行混合营养培养，可用于优化BDF生产。该策略通过甘油循环利用降低成本，并通过克服高密度培养中光穿透率降低导致的生长迟缓来提高脂质生产率和生物质生产率。
结果
在产油海洋硅藻Fistulifera solaris JPCC DA0580中过表达内源性甘油激酶（GK）基因，可加速甘油代谢并提高脂质和生物质生产率。基于生长情况和基因组整合确认，从90个G418抗性克隆中筛选出两个候选克隆。所选克隆（GK1_7和GK2_16）中的GK基因表达高于野生型培养物。GK2_16克隆的脂质生产率提高了12%。
结论
我们已经证明了在产油微藻中进行代谢工程以提高脂质生产率的潜力。代谢工程技术可用于优化BDF生产。
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