Luo Wei, Liu Chun-lei, Wang Jun-ying, Huang Chao, Yi Hui-juan
Zhen Ci Yan Jiu. 2014 Dec;39(6):482-6.
To observe the effect of electroacupuncture (EA) combined with cutaneous "Tongluo" (meridian- dredging) stimulation on gastric electrical activities and gastromucosal prostaglandin (PG) level in rats with chronic atrophic gastritis (CAG), so as to investigate its mechanism underlying improvement of gastric function.
A total of 40 SD rats were randomly divided into blank control, CAG model, medication and combined therapy groups, with 10 rats being in each group. CAG model was established by. intragastric administration of deionized water with N-methyl-N '-nitro-N-nitrosoguanidine (MNNG) solution (5 mL/kg), once a day, in combination with alternate fasting and full-eating and lavage of 2% sodium salicylate and warm saline (15%) for 12 weeks. For rats of the combined therapy group, EA was applied to "Zusanli" (ST 36), "Zhong- wan" (CV 12), "Tianshu" (ST 25) and "Pishu" (BL 20), two acupoints for one session, followed by cutaneous "Tongluo" stimulation of ST 25 and BL 20 by using an intelligent Tongluo therapeutic instrument for 20 min, once daily for 2 months. Electrogas- trography (EGG) was recorded using an intelligent gastrointestinal electrographic instrument. Gastromucosal PGE2 and PGF2, contents were assayed by radioimmunoassay.
compared to the control group, the mean frequency and amplitude of EGG, and gastromucosal PGE2 and PGF2a contents were obviously decreased while the abnormal rhythm index and frequency-variation- coefficiency levels were markedly increased in the model group (P<0. 01). Following treatment, the above-mentioned changes of the 6 indexes were significantly reversed by both medication and combined therapy (P<0. 01). No significant differences were found between the medication and combined therapy groups (P>0.05).
Combined use of EA and intelligent Tongluo stimulation can significantly improve the gastroelectric dysrhythmia in CAG rats, which may be closely associated with its effect in up-regulating gastric mucosal PGE2 and PGF2, levels.
观察电针（EA）联合皮肤“通络”（疏通经络）刺激对慢性萎缩性胃炎（CAG）大鼠胃电活动及胃黏膜前列腺素（PG）水平的影响，以探讨其改善胃功能的机制。
将40只SD大鼠随机分为空白对照组、CAG模型组、药物治疗组和联合治疗组，每组10只。通过胃内给予含N-甲基-N'-硝基-N-亚硝基胍（MNNG）溶液（5 mL/kg）的去离子水，每天1次，联合禁食与饱食交替以及用2%水杨酸钠和温盐水（15%）洗胃12周来建立CAG模型。联合治疗组大鼠，电针“足三里”（ST 36）、“中脘”（CV 12）、“天枢”（ST 25）和“脾俞”（BL 20），每次选两个穴位，然后用智能通络治疗仪对ST 25和BL 20进行皮肤“通络”刺激20分钟，每天1次，共2个月。使用智能胃肠电图仪记录胃电图（EGG）。采用放射免疫分析法检测胃黏膜PGE2和PGF2α含量。
与对照组相比，模型组EGG的平均频率和振幅以及胃黏膜PGE2和PGF2α含量明显降低，而异常节律指数和频率变异系数水平显著升高（P<0.01）。治疗后，药物治疗组和联合治疗组上述6项指标的变化均得到显著逆转（P<0.01）。药物治疗组和联合治疗组之间无显著差异（P>0.05）。
电针与智能通络刺激联合应用可显著改善CAG大鼠的胃电节律紊乱，这可能与其上调胃黏膜PGE2和PGF2α水平的作用密切相关。
