Zhang Hai-Feng, Luo Shu-Yu, Zhang Yao, Hu Bing, Li Lin-Hui, Zhang Bo, Ge Lai-An, Kang Ming-Fei, Fu Yong, Xiong Jun
Department of Acupuncture and Moxibustion, Affiliated Hospital of Jiangxi University of TCM, Nanchang 330006, China.
Graduate School of Jiangxi University of TCM.
Zhongguo Zhen Jiu. 2020 Mar 12;40(3):279-84. doi: 10.13703/j.0255-2930.20190224-k0002.
To observe the effect of heat-sensitive moxibustion at "Zhongwan" (CV 12) on serum growth hormone (GH) and pepsinogen (PG) in chronic atrophic gastritis (CAG) rats, and to explore the potential mechanism of heat-sensitive moxibustion for CAG.
A total of 66 male SD rats were randomized into a blank group (12 rats) and a model establishment group (54 rats). No intervention was given in the blank group. Rats in the model establishment group were intervented with compound pathogeny method for 12 weeks to establish CAG model, which were further divided into a model group (11 rats), a vitacoenzyme group (11 rats) and a moxibustion group (22 rats). In the moxibustion group, suspending moxibustion was applied at "Zhongwan" (CV 12) for 40 min. After the intervention of moxibustion, 0.9% sodium chloride solution was given by gavage (2 mL·kg·d). According to the changes of tail temperature, rats in the moxibustion group were divided into a heat-sensitive moxibustion group (11 rats) and a non-heat-sensitive moxibustion group (8 rats). The vitacoenzyme group was given vitacoenzyme as the same dose by gavage. The intervention was adopted once a day for 28 days. Changes of body weight were observed among the groups. Expressions of serum GH, PGⅠand PGⅡwere detected by ELISA, and the ratio of PGⅠand PGⅡ (PGR) was calculated. The morphological changes of gastric mucosa were observed by macroscopy and light microscope.
①After modeling, the body weight of rats in the model establishment group was lower than the blank group (<0.01). Compared with the model group, the body weight of rats in the vitacoenzyme group, the heat-sensitive moxibustion group and the non-heat-sensitive moxibustion group was increased after intervention (<0.05), and there were no significant differences among the intervention groups (>0.05). ②Under macroscopy and light microscope, gastric tissue of rats after modeling showed dark red and pale gastric mucosa, lower plica and mucosal congestion. The glands of lamina propria were atrophied or disappeared with sparse and disordered arrangement, in which, lymphoid follicles and inflammatory cells could be observed. After intervention, morphology of gastric mucosa was improved in the vitacoenzyme group, the heat-sensitive moxibustion group and the non-heat-sensitive moxibustion group. ③Compared with the blank group, the serum levels of GH, PGⅠ, PGⅡ and PGR were decreased in the model group (<0.05, <0.01). Compared with the model group, the serum levels of GH, PGⅠand PGⅡwere increased in the vitacoenzyme group, the heat-sensitive moxibustion group and the non-heat-sensitive moxibustion group (<0.05, <0.01), the levels of PGR were increased without statistical difference (>0.05). Compared with the vitacoenzyme group and the non-heat-sensitive moxibustion group, the serum levels of GH and PGⅠwere increased in the heat-sensitive moxibustion group (<0.05).
Heat-sensitive moxibustion at "Zhongwan" (CV 12) can improve the morphology of gastric mucosa in chronic atrophic gastritis rats, its mechanism may be related to the up-regulation of serum GH and PGⅠ.
观察“中脘”（CV 12）热敏灸对慢性萎缩性胃炎（CAG）大鼠血清生长激素（GH）和胃蛋白酶原（PG）的影响，探讨热敏灸治疗CAG的潜在机制。
将66只雄性SD大鼠随机分为空白组（12只）和造模组（54只）。空白组不做干预。造模组采用复合致病法干预12周建立CAG模型，再将其分为模型组（11只）、维酶素组（11只）和艾灸组（22只）。艾灸组于“中脘”（CV 12）行悬灸40分钟。艾灸干预后，以0.9%氯化钠溶液灌胃（2 mL·kg·d）。根据尾温变化，将艾灸组大鼠分为热敏灸组（11只）和非热敏灸组（8只）。维酶素组给予同剂量维酶素灌胃。每天干预1次，共28天。观察各组大鼠体重变化。采用ELISA法检测血清GH、PGⅠ和PGⅡ表达，并计算PGⅠ与PGⅡ比值（PGR）。通过大体及光镜观察胃黏膜形态变化。
①造模后，造模组大鼠体重低于空白组（<0.01）。与模型组比较，干预后维酶素组、热敏灸组和非热敏灸组大鼠体重增加（<0.05），各干预组间差异无统计学意义（>0.05）。②大体及光镜下，造模后大鼠胃组织胃黏膜暗红色、色泽变淡，皱襞变低、黏膜充血。固有层腺体萎缩或消失，排列稀疏紊乱，可见淋巴滤泡及炎性细胞。干预后，维酶素组、热敏灸组和非热敏灸组胃黏膜形态均有改善。③与空白组比较，模型组血清GH、PGⅠ、PGⅡ及PGR水平降低（<0.05，<0.01）。与模型组比较,维酶素组、热敏灸组和非热敏灸组血清GH、PGⅠ和PGⅡ水平升高（<0.05，<0.01），PGR水平升高但差异无统计学意义（>0.05）。与维酶素组和非热敏灸组比较，热敏灸组血清GH和PGⅠ水平升高（<0.05）。
“中脘”（CV 12）热敏灸可改善慢性萎缩性胃炎大鼠胃黏膜形态，其机制可能与上调血清GH和PGⅠ有关。
