Aksoy Ayse Nur, Kabil Kucur Suna, Batmaz Gonca, Gözükara Ilay, Aksoy Mehmet, Kurt Nezahat, Mammadov Renad
Department of Obsterics and Gynecology, Nenehatun Hospital, Erzurum, Turkey.
Gynecol Obstet Invest. 2015;79(2):119-25. doi: 10.1159/000367657. Epub 2015 Jan 27.
BACKGROUND/AIMS: To investigate the importance of antioxidant activity in infertility caused by cisplatin in rats.
Rats in cisplatin control (CG), Vitamin E + cisplatin (ECG), Vitamin C + cisplatin (CCG), Hippophae rhamnoides extract (HRE) + cisplatin (HRECG), and thiamine pyrophosphate (TPP) + cisplatin (TPPCG) groups were injected intraperitoneally (ip) with (100 mg/kg) Vitamin E, Vitamin C, HRE, and TPP, respectively. One hour later, ip cisplatin was administered (5 mg/kg), and then antioxidant medications were continued for 10 days. Cisplatin + Vitamin E (CEG-1), cisplatin + Vitamin C (CCG-1), cisplatin + HRE (CHREG-1), and cisplatin + TPP (TPPCG-1) rats received cisplatin (5 mg/kg, ip) and were kept for 10 days. At the end of that period, rats received antioxidant medications for 10 days. (n = 12, for each group). Six rats from each group were sacrificed. Ovaries were removed to measure malondialdehyde, total glutathione, glutathione S-transferase, and glutathione reductase levels. The remaining rats were kept in a suitable laboratory environment.
Cisplatin-induced oxidative stress was best prevented by HRE, Vitamin E, Vitamin C, and TPP, in that order. However, infertility caused by cisplatin was only prevented and treated by TPP.
Oxidative stress is not a major component in the pathogenesis of cisplatin-associated infertility.
背景/目的：研究抗氧化活性在顺铂所致大鼠不育症中的重要性。
顺铂对照组（CG）、维生素E + 顺铂组（ECG）、维生素C + 顺铂组（CCG）、沙棘提取物（HRE）+ 顺铂组（HRECG）和硫胺素焦磷酸（TPP）+ 顺铂组（TPPCG）的大鼠分别腹腔注射（100 mg/kg）维生素E、维生素C、HRE和TPP。1小时后，腹腔注射顺铂（5 mg/kg），然后持续给予抗氧化药物10天。顺铂 + 维生素E组（CEG - 1）、顺铂 + 维生素C组（CCG - 1）、顺铂 + HRE组（CHREG - 1）和顺铂 + TPP组（TPPCG - 1）的大鼠接受顺铂（5 mg/kg，腹腔注射）并饲养10天。在该时间段结束时，大鼠接受抗氧化药物治疗10天。（每组n = 12）。每组处死6只大鼠。取出卵巢以测量丙二醛、总谷胱甘肽、谷胱甘肽S - 转移酶和谷胱甘肽还原酶水平。其余大鼠饲养在合适的实验室环境中。
顺铂诱导的氧化应激依次被HRE、维生素E、维生素C和TPP最佳地预防。然而，顺铂所致的不育症仅被TPP预防和治疗。
氧化应激不是顺铂相关性不育症发病机制中的主要因素。
