Haddad Nizar Jamal
a Bee Research Department , National Center for Agricultural Research and Extension , Amman , Jordan .
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4067-4068. doi: 10.3109/19401736.2014.1003846. Epub 2015 Jan 30.
The mitochondrial genome sequence of Levant Region honeybee, Apis mellifera syriaca, is analyzed and presented for the public for the first time. The genome of this honeybee is 15,428 bp in its length, containing 13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes. The overall base composition is A (42.88%), C (9.97%), G (5.85%), and T (41.3%), the percentage of A and T being higher than that of G and C. Percentage of non-ATGC characters is 0.007. All the genes are encoded on H-strand, except for four subunit genes (ND1, ND4, ND4L, and ND5), two rRNA genes and eight tRNA genes. The publication of the mitochondrial genome sequence will play a vital role in the conservation genetic projects of A. mellifera, in general, and Apis mellifera syriaca, in particular; moreover, it will be useful for further phylogenetic analysis.
首次对黎凡特地区蜜蜂(叙利亚蜜蜂,Apis mellifera syriaca)的线粒体基因组序列进行了分析并向公众公布。这种蜜蜂的基因组长度为15428 bp,包含13个蛋白质编码基因、22个转运RNA基因和2个核糖体RNA基因。总体碱基组成为A(42.88%)、C(9.97%)、G(5.85%)和T(41.3%),A和T的百分比高于G和C。非ATGC字符的百分比为0.007。除了四个亚基基因(ND1、ND4、ND4L和ND5)、两个rRNA基因和八个tRNA基因外,所有基因均在H链上编码。线粒体基因组序列的公布将对一般蜜蜂尤其是叙利亚蜜蜂的保护遗传项目起到至关重要的作用;此外,它将有助于进一步的系统发育分析。