谷氨酰胺酶抑制与神经递质谷氨酸从突触体的释放。
Glutaminase inhibition and the release of neurotransmitter glutamate from synaptosomes.
作者信息
Bradford H F, Ward H K, Foley P
机构信息
Department of Biochemistry, Imperial College of Science and Technology, London, U.K.
出版信息
Brain Res. 1989 Jan 2;476(1):29-34. doi: 10.1016/0006-8993(89)91533-3.
Cerebrocortical synaptosomes were incubated with glutamine together with 6-diazo-5-oxo-L-norleucine (DON) and NH4Cl (which are known to inhibit phosphate-stimulated glutaminase) in order to assess the effect of such inhibition on the pool sizes and extent of evoked release of endogenous amino acids, particularly glutamate. DON (5 mM) inhibited glutaminase by 73-89% and NH4Cl (1-4 mM) inhibited the enzyme by 45-53% under the conditions employed. NH4Cl (4 mM) incubated with synaptosomes for 30 min reduced pool sizes of aspartate and glutamate by 28% and inhibited release of glutamate by 55% compared to control release. DON caused a decrease in both the pool size of glutamate (22%) and the extent of veratrine-evoked release of amino acids (21%).
将大脑皮质突触体与谷氨酰胺、6-重氮-5-氧代-L-正亮氨酸（DON）和氯化铵（已知可抑制磷酸盐刺激的谷氨酰胺酶）一起孵育，以评估这种抑制作用对内源性氨基酸（尤其是谷氨酸）的库容量和诱发释放程度的影响。在所采用的条件下，5 mM的DON抑制谷氨酰胺酶73%-89%，1-4 mM的氯化铵抑制该酶45%-53%。与对照释放相比，4 mM氯化铵与突触体孵育30分钟使天冬氨酸和谷氨酸的库容量减少28%，并抑制谷氨酸释放55%。DON使谷氨酸的库容量减少22%，并使藜芦碱诱发的氨基酸释放程度降低21%。
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