Fox B C, Mobley H L, Wade J C
Section of Infectious Diseases and Microbiology, University of Maryland Cancer Center, Baltimore 21201.
J Infect Dis. 1989 Mar;159(3):488-94. doi: 10.1093/infdis/159.3.488.
Reproducible typing procedures to differentiate isolates of Candida albicans are limited. C. albicans isolates were obtained from immunocompromised patients by using DNA restriction enzyme fragment analysis and hybridization with both a radiolabeled mitochondrial DNA probe and a nonradioactive (biotinylated) DNA probe. There were 110 pathogenic and nonpathogenic C. albicans isolates from 63 immunocompromised patients. EcoRI restriction fragment analysis with the biotinylated probe revealed different "fingerprint" patterns for 60 of 63 patients. Analysis of 57 isolates from 20 patients showed no intrapatient variation regardless of the isolation site. DNA probe "fingerprint" patterns were analyzed for eight patients on serially recovered (range, 2-18 mo) C. albicans isolates. The unique patient profiles persisted over time. The application of this biotinylated C. albicans DNA probe provides a more sensitive means than simple gel restriction fragment analysis to define the epidemiology of C. albicans infection. The use of this biotin-labeled nonradioactive probe has potential application in clinical evaluations of outbreaks of nosocomial candidiasis.
用于区分白色念珠菌分离株的可重复分型程序有限。通过DNA限制性内切酶片段分析以及与放射性标记的线粒体DNA探针和非放射性（生物素化）DNA探针杂交，从免疫功能低下的患者中获得白色念珠菌分离株。共有来自63名免疫功能低下患者的110株致病性和非致病性白色念珠菌分离株。使用生物素化探针进行的EcoRI限制性片段分析显示，63名患者中有60名呈现出不同的“指纹”模式。对来自20名患者的57株分离株进行分析表明，无论分离部位如何，患者体内均无变异。对8名患者连续回收（时间范围为2 - 18个月）的白色念珠菌分离株进行了DNA探针“指纹”模式分析。独特的患者图谱随时间持续存在。这种生物素化的白色念珠菌DNA探针的应用提供了一种比简单的凝胶限制性片段分析更敏感的方法来确定白色念珠菌感染的流行病学。这种生物素标记的非放射性探针在医院念珠菌病暴发的临床评估中具有潜在应用价值。
